Evaluation of mitochondrial activity in mature bovine oocytes during their aging in vitro
Abstract
Aging in in vitro-matured oocytes negatively affects its fitness. However, there are few data on aging-related changes in oocytes.. In this research for was to determine the mitochondrial pattern of distribution of the matured bovine oocytes during their prolonged culture in vitro. Bovine cumulus-oocyte complexes (COCs) were cultured for 20 h in maturation medium (TCM 199 supplemented with 10% fetal calf serum (FCS), 10 μg/ml FSH, and 10 μg/ml LH). After IVM, COCs and oocytes denuded of their CCs (DO) were transferred to the aging medium consisting of TCM 199 supplemented with 10% fetal calf serum and prolonged cultured for 12 h. After maturation (20 h) or prolonged culture MII oocytes (12 h) were studied to determine mitochondrial activity using MitoTracker Orange fluorescence. For oocytes analyzed just after IVM mitochondrial activity was low, while after 12 h prolonged culture of mature bovine oocytes the activity increased (P<0,05). At the same time the removal of CCs before prolonged culture affected mitochondrial activity reducing (P<0,05). The result obtained point to increasing the level of mitochondrial activity during in vitro aging of bovine oocytes and to that the removal of CCs is able to decelerate these age-associated alterations.
About the Authors
A. .. Lopukhov
L.K. Ernst Federal Science Center for Animal Husbandry
Russian Federation
Russian Federation
E. .. Shedova
L.K. Ernst Federal Science Center for Animal Husbandry
Russian Federation
Russian Federation
E. .. Tsyndrina
L.K. Ernst Federal Science Center for Animal Husbandry
Russian Federation
Russian Federation
G. .. Singina
L.K. Ernst Federal Science Center for Animal Husbandry
Russian Federation
Russian Federation
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Review
For citations:
Lopukhov A..., Shedova E..., Tsyndrina E..., Singina G... Evaluation of mitochondrial activity in mature bovine oocytes during their aging in vitro. Genetics and breeding of animals. 2018;(1):33-36. (In Russ.)
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