To study the effect of gene polymorphism MLN bulls of Holstein breed on the frequency of occurrence of displacement of abomasum in the offspring

Abstract

The displacement of abomasum is disease characterized by a change in the position of authority and a breach of its functionality. Studies linking certain areas of the genome of cattle with the manifestation of this pathology continue to be carried out until the results are quite contradictory. The goal of this research was to study the effect of polymorphism FN298674: g. 90 T → C the MLN gene (gene motilin) bulls of Holstein breed on the frequency of occurrence of displacement of abomasum in their offspring. We have designed a test system for DNA testing of mutation. The test system is based on AC-PCR (allele specific polymerase chain reaction). Genotyping by MLN locus (the gene of the hormone motilin) of a sample of bulls (n=34) of Holstein breed (World Wide Sires) was carried out. There were no significant differences between the observed and expected distribution of genotypes. Each of the 34 genotyped bulls was evaluated by the quality of the offspring (estimated December 2017, the number of daughters - 17718, the number of herds - 6048), with the definition of the risk index of displacement of the stench in daughters. The range of index values in genotyped bulls varied in the range of values from 96 to 109. The study did not reveal a reliable relationship between the risk index of displacement of the stench in daughters and the father's MLN genotype, which may indicate the absence of such a relationship. The obtained data require follow-up studies.