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Features of growth and development in chicken embryos under the influence of tissue-specific expression of integrated recombinant genes

Abstract

The influence of integration and expression of recombinant genes on the growth and development in chicken embryos was studied. The subject of the study was embryos, which were obtained from transgenic chickens with tissue-specific expression of the integrated marker gene eGFP. For the tissue-specific expression of the integrated gene, the regulatory elements of the ovalbumin gene in combination with the promoter enhancers CMV (human cytomegalovirus) and Mo-MLV (leukemia virus of Moloney mice) were used. The embryos obtained from non-transgenic hens were used as a control. Weighed and linear indicators of growth and development in embryos on the 7th, 10th, 14th and 18th days of incubation were investigated. The influence of transgenesis on the intensity of growth and development in chicken embryos was established. Weight loss was observed in transgenic embryos compared to control. Differences between experimental groups by weight of embryos were the most significant in the second half of embryogenesis and reached 13% on day 14 of incubation, 17% on day 18 of incubation. A similar trend was observed in the linear indices of embryo development. A shorter body length than the control characterized transgenic embryos: these differences reached 4,1; 5,4; 8,3 and 7,8% on the 7th, 10th 14th and 18th days of incubation, respectively. The established differences in weight and linear indices between the experimental groups indicate the negative effect of integrated recombinant genes on the growth and development in chicken embryos. The most significant changes in the studied parameters were found in transgenic embryos with an integrated eGFP gene under the control of the ovalbumin gene promoter and the promoter-enhancer of the Mo-MLV gene.

About the Authors

A. N. Vetokh
L. K. Ernst Federal Science Center for Animal Husbandry
Russian Federation


N. A. Volkova
L. K. Ernst Federal Science Center for Animal Husbandry
Russian Federation


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Review

For citations:


Vetokh A.N., Volkova N.A. Features of growth and development in chicken embryos under the influence of tissue-specific expression of integrated recombinant genes. Genetics and breeding of animals. 2018;(3):22-26. (In Russ.)

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ISSN 2410-2733 (Print)