The effect of epidermal growth factor on quality of bovine oocytes aged in vitro
Abstract
Effect of epidermal growth factor (EGF) on modifications of metaphase-II (M-II) chromosomes in aged bovine oocytes and their developmental competence after in vitro fertilization was investigated in the present work. To this, the matured in vitro (for 20 h) cumulus-enclosed oocytes (GEOs) cultured for additional 24 or 12 h in the absence (Control) and in presence of EGF (1-500 ng/ml) and then used respectively for cytogenetic analysis or for in vitro fertilization. In the Control the rate of GEOs with destructive modifications of M-II chromosomes was 58.3 ± 3.2 %. EGF did not affect on the frequency of abnormal chromosome modifications at concentrations of 1-50 ng/ml and increased this frequency at the concentration 500 ng/ml. After the 12 h prolonged culture the cleavage rate of fertilized oocytes did not differ between groups, varying from71.5 to 76.5%. Meanwhile the addition of EGF at concentration of 10 ng/ml to the aging medium led to an increase in the yield of blastocysts from 9.7 ± 1.1 % (Control) to 18.2 ± 1.5 % (p<0.01). The data suggest that EGF is able to improve the competence for development of in vitro ageing bovine oocytes and that this effect of EGF is not related to the modeling of chromosome quality.
About the Authors
G. .. Singina
Federal Science Center for Animal Husbandry named after Academy Member L. K. Ernst
Russian Federation
Russian Federation
E. .. Shedova
Federal Science Center for Animal Husbandry named after Academy Member L. K. Ernst
Russian Federation
Russian Federation
I. .. Lebedeva
Federal Science Center for Animal Husbandry named after Academy Member L. K. Ernst
Russian Federation
Russian Federation
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Singina G..., Shedova E..., Lebedeva I... The effect of epidermal growth factor on quality of bovine oocytes aged in vitro. Genetics and breeding of animals. 2018;(4):29-33. (In Russ.)
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