Selection of cattle for polymorphic beta-casein gene in the Krasnodar Territory

Abstract

The problem of production of safe for the consumer dairy products is quite relevant. Studies have been conducted to study the effect of beta-casein gene polymorphism (CSN2) on the safety of milk and dairy products. It was found that there is a relationship between the consumption of milk β-casein type A1 and various diseases. The purpose of our research - to develop an effective author's test system, affordable for the consumer, optimal for having in the laboratory equipment and to establish using the developed test system the frequency of occurrence of polymorphic variants in a sufficient array of livestock to allocate a group of animals-carriers of the genotype A2A2 for the production of A2 milk. The PCR/RPF method was used in the development of the test system. Primers are selected using the program Primer Premier, amplification conditions-empirically. To create a restriction site in the area of the required SNP (T →G), an introduction to one of the nucleotide replacement primers was required. For conducting RFLP analysis used a restriction enzyme BstDEI. Testing of the test system was carried out using the DNA of bulls listed on the website https://www.accelgen.com CSN2 genotypes. The object of research was cows Ayrshire (n=685) and Holstein (n=1081) of JSC "Agroalimenta "Kuban". Total genotyped 1766 heads. It was found that allele A2 occurs at a frequency of 0.41 and 0.48, respectively, in animals of Ayrshire and Holstein breeds. Frequency of "desirable" А2А2 genotype was 0.19 and 0.21 in Ayrshire - Holstein breed of cattle.