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Effect of vitrification on the chromatin status in Sus Scrofa Domesticus cumulus - oocyte complexes

Abstract

Successful solution of the problem of preserving the viability of devitrified donor porcine oocytes will significantly intensify innovative cellular reproductive and DNA technologies by creating cryobanks of female gametes with positive qualitative characteristics. Creation an effective model of vitrification and media for the cultivation of frozen-thawed oocyte-cumulus complexes is an important task of cryo-and embryo-technologists. Highly dispersed silica nanoparticles (nHDS) possess, due to their structural features, a number of important properties (high sorption capacity of proteins, absence of allergenic and toxic effects on cells, activation of reparative processes, antioxidant effects), can be proposed as potential components of cryoprotectants and media for culturing devitrified cells. The effects of 0.001% nHDS on the chromatin status of native and devitrified Sus Scrofa Domesticus cumulus -oocyte complexes during in vitro culture have identified. 0.001% nHDS had not cyto- and genotoxic effects on both native and devitrified oocyte-cumulus complexes during cultivation, moreover, they reduced the level of destructive processes (apoptosis, pycnosis) provoked by ultralow temperatures (vitrification). A cryoprotective effect of 0.001% nHDS on devitrified somatic (cumulus) cells and gametes of Sus Scrofa Domesticus ovarian follicles after the freezing / thawing and cultivation during 44 hours was detected.The addition of 0.001% nHDS into cryoprotective and culture media had a positive effect on the morphofunctional state of cumulus cells after the vitrification and cultivation procedure. The proportion of cells with compact cumulus treated with 0.001% nHDS after devitrification increases from 33 % to 48 %, P<0,01. The level of oocytes with cumulus in a high degree of expansion after 44 hours of cultivation with 0.001% nHDS was 61 % vs 39 %, P<0,05, in control. The use of nHDS in the steps of the cumulus- oocyte- complexes vitrification procedure caused a decrease in the proportion of apoptotic cells (TUNEL-test) in the cumulus from 54 % to 35 %, P<0,05. The level of matured devitrified oocytes treated with 0.001% nHDS before culture increased from 33 % control to 59 %, P<0,001. The obtained results allow us to recommend the use of 0.001% nHDS in the steps of porcine oocytes vitrification technology.

About the Authors

T. I. Kuzmina
Russian Research Institute of Farm Animal Genetics and Breeding - Branch of the L.K. Ernst Federal Science Center for Animal Husbandry
Russian Federation


T. I. Stanislavovich
Russian Research Institute of Farm Animal Genetics and Breeding - Branch of the L.K. Ernst Federal Science Center for Animal Husbandry
Russian Federation


References

1. Khalili M. A. Vitrification of human immature oocytes before and after in vitro maturation: a review / M. A. Khalili, А. Shahedi, S. A. Nottola, S. Ashourzadeh, G. Macchiarelli, M. G. Palmerini, J. Assist // Reprod. Genet. - 2017. - V.34. - P.1413-1426.

2. Kuwayama M. Vitrifacation of human oocyte // Reproductive BioMedcine. - 2010. - V.20. - P.538-539.

3. Ogawa B. Developmental ability of porcine in vitro matured oocytes at the meiosis II stage after vitrification / B. Ogawa, S. Ueno, N. Nakayama, H. Matsunari, K. Nakano, T. Fujiwara, Y. Ikezawa, H. Nagashima // J Reprod Dev. - 2010. - V.56. - P.356-361.

4. Kikuchi K. Morphological features of lipid droplet transition during porcine oocyte fertilisation and early embryonic development to blastocyst in vivo and in vitro / K. Kikuchi, H. Ekwall, P. Tienthai, Y. Kawai, J. Noguchi, H. Kaneko and H. Rodriguez-Martinez // Zygote. - 2002. - V.10. - P.355-366.

5. Novichkova D. Effects of highly dispersed silica nanoparticleson morphology of lipid droplets in growing or fully grown porcine oocytes / D. Novichkova, T. Kuzmina // Reproduction in Domestic Animals. - 2018. - V.53. - P.173-174.

6. Новичкова Д. А. Воздействие кремнийсодержащих соединений на липидом ооцитов Sus scrofa domesticus / Д. А. Новичкова, Т. И. Кузьмина, Т. Г. Хонина // Технологии живых систем. - 2018. - №5. - С.58-63.

7. Nagashima H. Removal of cytoplasmic lipid enhances the tolerance of porcine embryos to chilling / H. Nagashima, N. Kashiwazaki, R.J. Ashman, C.G. Grupen, R.F. Seamark, M.B. Nottle // Biol Reprod. - 1994. - V.51. - P.618-622.

8. Галаган Н. П. Биофункциональные наноматериалы на основе высокодисперсного кремнезема, белка и аминоуглеводов / Н. П. Галаган, Н. Ю. Клименко, И. Л. Орел, Е. А. Новикова, В. В. Туров // Biopolymers and Cell. - 2010. - Т.26(3). - С.205-213.

9. Геращенко И. И. Мембранотропные свойства наноразмерного кремнезема // Поверхность. - 2009. - №.1.(16). - С.288-306.

10. Савченко Д. С. Изучение антиоксидантных свойств нанокомпозита высокодисперсного кремнезема с наночастицами серебра / Д. С. Савченко // Медицина и образование в Сибири. - 2013. - №6. - С.16.

11. Kuzmina T. I. Cytoprotective effect of highly dispersed silica nanoparticles on the viability of granulosa from porcine follicles / T. I. Kuzmina, T. I. Stanislavovich, V. Yu. Kravtsov // Russian Journal of Developmental Biology. - 2018. - V.49. - №4S. - P.22-23.

12. Кузьмина Т. И. Воздействие наночастиц высокодисперсного кремнезема на хроматин соматических клеток фолликулов свиньи / Т. И. Кузьмина, Д. А. Новичкова, И. В. Чистякова, О. А. Епишко // Ветеринария. - 2017. - №2. - С.43-45.

13. Бойцева Е. Н. Влияние наночастиц высокодисперсного кремнезема на апоптоз сперматозоидов Bos Taurus / Е. Н. Бойцева, Н. В. Бычкова, Т. И. Кузьмина // Цитология. - 2017. - Т.59. - №5. - С.375-380.

14. Настасієнко Н. С. Дослідження біологічної активності кремнеземів, модифікованих дитатриметилсілільними групамиі сорбітом, повідношенню до сперматозоїдів биків методом фотон-кореляційної спектроскопії / Н. С. Настасієнко, П. О. Кузема, Н. П. Галаган, В. А. Покровський // Фізика живого. - 2010. - Т.18. - №3. - С.99-106.

15. Зюзюн А. Б. Застосування наноматеріалу в ембріогенетичній системі in vitro отримання ембріонів свиней / А. Б. Зюзюн, О. В. Щербак, О. С. Осипчук, С. І. Ковтун, В. В. Дзіцюк // Фактори експериментальної еволюції організмів. - 2015. - Т.17. - С.164-168.

16. Кузьмина Т. И. Моделирование систем созревания ооцитов свиней in vitro / Т.И. Кузьмина, Д.А. Новичкова, Н.А. Волкова // Сельскохозяйственная биология. - 2013. - №2. - С.52-57.

17. Tarkowski A. An air-diying method for chromosomal preparation from mouse eggs / A. Tarkowski // Cytogenetic. - 1966. - V.1. - P.394-400.

18. Eppig John J. The mammalian oocyte orchestrates the rate of ovarian follicular development / John J. Eppig, К. Wigglesworth and Frank L. Pendola // Developmental Biology. - 2002. - V.99. - №.5. - P.2890-2894.


Review

For citations:


Kuzmina T.I., Stanislavovich T.I. Effect of vitrification on the chromatin status in Sus Scrofa Domesticus cumulus - oocyte complexes. Genetics and breeding of animals. 2019;(1):27-33. (In Russ.)

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ISSN 2410-2733 (Print)