Electrocardiography in rats in experimental studies (review of the literature)
https://doi.org/10.31043/2410-2733-2019-2-108-120
Abstract
A scientific and analytical review of experimental research with the use of rats as biological test systems and electrocardiogram (ECG) parameters assessment was conducted. The data on various methods of ECG recording in anesthetized and unanesthetized animals (including the advantages and drawbacks of such methods) were presented. Both invasive and non-invasive approaches to recording the cardiac electrical activity in unanesthetized rats (including the method of telemetric ECG monitoring), which allow EEG monitoring in freely-behaving animals, were described. The review widely presents the studies with ECG registration in anesthetized animals. The data on influence of various types of anesthesia on electrical manifestation of cardiac activity in rats as well as hemodynamic parameters and activity of the vegetative centers regulating them was systematized. The review analyzes the parameters of rat’s ECG recorded in experimental studies in comparison with the parameters of human’s ECG and assesses the possibilities of extrapolating the data obtained to humans. The literature data analysis revealed a fairly wide range of scientific studies and preclinical trials of drugs based on the use of the electrocardiography method in rats. The most significant ECG parameters in rats were identified: PR interval, QRS complex (RS or Rs), T wave amplitude and corrected QT interval, which are of practical importance for adequate interpretation of the data of experimental research of integral characteristics of cardiovascular system and intersystem regulatory mechanisms activity.
About the Authors
I. Privalova
FSBEI HE Kursk State Medical University
Russian Federation
Russian Federation
PhD (Biol. Sci.), Professor of the Department of normal physiology
305041, Kursk region, Kursk, st. K. Marx, 3
O. Shevelev
Peoples’ Friendship University of Russia (RUDN University)
Russian Federation
Russian Federation
PhD (Med. Sci.), Professor of the Department of pathological physiology
117198, Moscow, ul. Miklouho-Maclay, 6
N. Hodorovich
Peoples’ Friendship University of Russia (RUDN University)
Russian Federation
Russian Federation
PhD (Med. Sci.), Professor of the Department of pathological physiology
117198, Moscow, ul. Miklouho-Maclay, 6
T. Kuznetsova
FSBEI HE «St. Petersburg State Academy of Veterinary Medicine»
Russian Federation
Russian Federation
PhD (Biol. Sci.), assistant of the Department of veterinary genetics and animal husbandry
196084, St. Petersburg, Chernigovskaya ul, 5
I. Glotova
FSBEI HE Kursk State Medical University
Russian Federation
Russian Federation
student
305041, Kursk region, Kursk, st. K. Marx, 3
T. Legostaeva
FSBEI HE Kursk State Medical University
Russian Federation
Russian Federation
student
305041, Kursk region, Kursk, st. K. Marx, 3
I. Ozerova
FSBEI HE Kursk State Medical University
Russian Federation
Russian Federation
student
305041, Kursk region, Kursk, st. K. Marx, 3
References
1. He J. P-Wave Indices and Risk of Ischemic Stroke: A Systematic Review and Meta-Analysis / J. He, G. Tse, P. Korantzopoulos, K. Letsas, S. Ali-Hasan-Al-Saegh, H. Kamel, G. Li, G. Lip, T. Liu // Stroke. — 2017. — Vol. 48. — ‹ 8. — P. 2066–2072.
2. Popadyuk V. I., Ilinskaya M. V., Shevelev O. A. Tonsilectomy and heart rate variability: stress assessment // Ecological and physiological problems of adaptation. Proceedings of the XVII All-Russian Symposium. — 2017. — P. 178–180.
3. Shvets D. A. Diagnostic value and mechanisms of postsystolic shortening in case of post-infarction focal changes in the left ventricle / D. A. Shvets, S. V. Povetkin // Man and His Health. — 2015. — ‹ 1. — P. 59–64.
4. Karkishchenko N. N. Basics of biomodeling. — M .: MIC, 2005. 608 p.
5. Kuryanova Ye. V. Effect of anesthesia and reflex stimulation of the sympathoadrenal and parasympathetic systems on heart rate variability in rats / E. V. Kuryanova // Natural Sciences. Journal of Fundamental and Applied Research. — 2011. — ‹2 (35). — P. 140–148.
6. Dor-Haim H. Redused ventricular arrhythmogeneity and increased electrical complexity in norm al exercised rats / H. Dor-Haim, O. Berenfeld, M. Horowitz, S. Lotan, M. Swissa // PLoS One. — 2013. — Vol. 8. — ‹ 6. — P. 168–176.
7. Pereira-Junior P. P. Noninvasive method for electrocardiogram recording in conscious rats: feasibility for heart rate variability analysis / P. P. Pereira-Junior, M. Marocolo, F. P. Rodrigues, E. Medei, J. H. Nascimento // An Acad Bras Cienc. 2010. — Vol. 82. — ‹ 2. — P. 431–437.
8. O. V. Sergeeva, N. N. Alipov, V. M. Smirnov. Vliyanie atropina, propranolola i atenolola na volnovuyu strukturu kolebanij ritma serdca u krys [Influence of atropine, propranolol and atenolol on the wave structure of heart rate fluctuations in rats] // Byulleten' ehksperimental'noj biologii i mediciny [Bulletin of Experimental Biology and Medicine]. 2008. T. 145. ‹ 4. S. 364–367.
9. Ryzhakina A. YU., Oshevenskij L. V., Krylov V. N. Vliyanie svetovogo rezhima vivariya na variabel'nost' serdechnogo ritma krys [Influence of the light regime of the vivarium on the variability of the heart rhythm of rats] // Vestnik Nizhegorodskogo universiteta im. N.I. Lobachevskogo [Bulletin of the Nizhny Novgorod University. N. I. Lobachevsky]. 2014. ‹ 4 (1). P. 183–187.
10. Damasceno D. Cardiac dysfunction in rats prone to audiogenic epileptic seizures / D. Damasceno, Q. Savergnini, E. Gomes, S. Guatimosim, J. Ferreira, C. Doretto, P. Almeida // Seizure. — 2013. — Vol. 22. — P. 259–266.
11. Fazan Jr. R. Evaluation of cardiovascular risk factors in the Wistar audiogenic rat (WAR) strain / Jr. R. Fazan, C. Silva, J. Oliveira, H. Salgado, N. Montano, N. Garcia-Cairasco // PLoS One. — 2015.
12. Es'haghi F. Investigation of ECG changes in absence epilepsy on WAG/ rij rats / F. Es'haghi, P. Shahabi, J. Frounchi, M. Sadighi, H. Yousefi // Basic and Clinical. — 2015. — Vol. 6. — ‹ 2.
13. Carll A. Diesel exhaust inhalation increases cardiac output, bradyarrhythmias, and parasympathetic tone in aged heart failure–prone rats / A. Carll, R. Lust, M. Hazari, C. Perez, Q. Krantz, C. King, D. Winsett, W. Cascio, D. Costa, A. Farraj // Toxicological sciences. — 2013. — Vol. 131. — ‹ 2. — P. 583–595.
14. Rey M. Simultaneous pulmonary and systemic blood pressure and ECG interval measurement in conscious, freely moving rats / M. Rey, E. Weber, P. Hess // Journal of the American Association for Laboratory Animal Science. — 2012. — Vol. 51. — ‹ 2. — P. 231–238.
15. Carll A. Whole and particle-free diesel exhausts differentially affect cardiac electrophysiology, blood pressure, and autonomic balance in heart failure–prone rats / A. Carll, R. Lust, M. Hazari, C. Perez, Q. Krantz, C. King, D. Winsett, D. Costa, A. Farraj // Toxicological sciences. — 2012. — Vol. 128. — ‹2. — P. 490–499.
16. Scridon A. Unprovoked atrial tachyarrhythmias in aging spontaneously hypertensive rats: the role of the autonomic nervous system / A. Scridon, C. Gallet, M. Arisha, V. OrÈa, B. Chapuis, N. Li, A. Tabib, G. ChristÈ, C. BarrËs, C. Julien, P. Chevalier // American journal of physiology. Heart and circulatory physiology. — 2012. — P. 386–392.
17. Zapadnyuk I. P., Zahariya E. A. Laboratornye zhivotnye. Razvedenie, soderzhanie, isspol'zovanie v ehksperimente: uchebnoe posobie [Laboratory animals. Breeding, content, use in the experiment: a tutorial]. — Kiev: Vishcha shkola, 1983. 383 p.
18. Konopelski P. Electrocardiography rats a comparison to human / P. Konopelski, M. Ufnal // Physiol Res. — 2016. — Vol. 65. — ‹ 5. — P. 717–725.
19. Jamali F. Methoxyflurane anesthesia augments the chronotropic and dromotropic effects of verapamil / F. Jamali, P. R. Mayo // J Pharm Sci. — 1999. — Vol. 2. — ‹ 1. — P. 30–35.
20. Hacker S. O. A comparison of target-controlled infusion versus volatile inhalant anesthesia for heart rate, respiratory rate, and recovery time in a rat model / S. O. Hacker, C. E. White, I. H. Black // Contemp Top Lab Anim Sci. — 2005. — Vol. 44. — ‹ 5. — P. 7–12.
21. Zhao Y. The quaternary lidocaine derivative QX-314 produces long-lasting intravenous regional anesthesia in rats / Y. Zhao, C. Zhou, J. Liu, P. Liang, D. Liao, Y. Chen, X. Chen // PLoS One. — 2014. — Vol. 9. — ‹ 6. — P. 34–41.
22. Haworth R. Inhibition of sodium/calcium exchange and calcium channels of heart cells by volatile anesthetics / R. Haworth, A. Goknur // Anesthesiology. — 1995. — ‹82. — P. 1255–1265.
23. Albrecht M. Effects of isoflurane, ketamine-xylazine and a combination of medetomidine, midazolam and fentanyl on physiological variables continuously measured by telemetry in Wistar rats / M. Albrecht, J. Henke, S. Tacke, M. Markert, B. Guth // BMC Veterinary Research. — 2014. — Vol. 198. — ‹ 10. — P. 95–103.
24. Regan C. In vivo cardiac electrophysiologic and antiarrhythmic effects of an isoquinoline IKur blocker, ISQ-1, in rat, dog, and nonhuman primate / C. Regan, G. Stump, A. Wallace, K. Anderson, C. Mcintyre, N. Liverton, J. J. Lynch // J Cardiovasc Pharmacol. — 2007. — ‹49. — P. 236–245.
25. Miranda A. Time course of echocardiographic and electrocardiographic parameters in myocardial infarct in rats / A. Miranda, R. Costa-e-Sousa, J. Werneck-de-Castro, E. Mattos, E. Olivares, V. Ribeiro, M. Silva, R. Goldenberg, A. Campos-de-Carvalho // An Acad Bras Cienc. — 2007. — ‹ 79. — P. 639–648.
26. Ziyatdinova N.I., Biktemirova R.G., Hisamieva L.I., Zefirov T.L. Vozrastnye osobennosti adrenergicheskoj regulyacii hronotropnoj funkcii serdca krys [Age features of adrenergic regulation of the chronotropic function of the heart of rats] // Zdorov'e, demografiya, ehkologiya finno-ugorskih narodov [Health, demography, ecology of Finno-Ugric peoples]. 2015. ‹ 4.P. 34–36.
27. Krzemiński T. F. Differential effects of four xylidine derivatives in the model of ischemia- and re-perfusion-induced arrhythmias in rats in vivo / T. F. Krzemiński, K. Mitrega, M. Zorniak, M. Porc // Eur J Pharmacol. — 2010. — Vol. 644. — ‹1-3. — P. 120–127.
28. Ahmad A. Impact of Isoprenaline and Caffeine on development of left ventricular hypertrophy and renal hemodynamic in Wistar Kyoto rats / A. Ahmad, M. Sattar, H. Rathore, S. Khan, M. Lazhari, F. Hashmi, N. Abdullah, E. Johns // Acta Pol Pharm. — 2015. — Vol. 72. — ‹ 5. — P. 1015–1026.
29. Makhmudova L. Kh. Functional biochemical and histological indicators of myocardium in stress-induced cardiac abnormalities / L. Kh. Makhmudova // Bulletin of the Russian Academy of Medical Sciences. — 2009. — T. 139. — ‹ 5. — P. 113–116.
30. Trofimenko A. I. Features of the electrocardiogram in rats with a model of cerebral ischemia caused by coagulation of the right middle cerebral artery / A. I. Trofimenko, A. H. Kade, V. P. Lebedev, S. A. Zanin, T. B. Zabolotskikh, S. N. Merzlyakova, M. V. Kolpakov // Kuban Scientific Medical Herald. — 2012. — V. 131. — 2. — P. 175–179.
31. Privalova I. L. Analysis of parameters of the electrical activity of the heart of rats under conditions of modeling of acetate ulcers / I. L. Privalova, E. T. Kamal, I. V. Glotova // Health and Education in the 21st Century. — 2017. — T. 19. — ‹4. — P. 120–122.
32. DIRECTORY. Physiological, biochemical and biometric indicators of the norm of experimental animals (edited by Makarov V. G., Makarova M. N.) St. Petersburg: Publishing house «LEMA», 2013. — 116 p.
33. Belousov M. V. Study of the cardiotoxic properties of native humic acids of peat / M. V. Belousov, R. R. Akhmedzhanov, M. V. Zykova, L. N. Maslov, A. N. Guriev, M. S. Yusubov // Bulletin of Siberian medicine. — 2014. — í.13. — ‹ 1. — P. 14–19.
34. Regan C. Novel method to assess cardiac electrophysiology in the rat: characterization of standard ion channel blockers / C. Regan, H. Cresswell, R. Zhang, J. Lynch // J Cardiovasc Pharmacol. — 2005. — ‹ 46. — P. 68–75.
35. Atli O. Evaluation of azithromycin induced cardiotoxicity in rats / O. Atli, S. Ilgin, H. Altuntas, D. Burukoglu // Int J Clin Exp Med. — 2015. — ‹ 8. — P. 3681–3690.
36. Ermolaev P. A., Hramyh T. P., Barskaya L. O. Izmenenie pokazatelej ehlektrokardiogrammy posle predel'no dopustimoj rezekcii pecheni u krys [Changes in the electrocardiogram after maximum permissible liver resection in rats] // Sibirskij medicinskij zhurnal (Irkutsk) [Siberian Medical Journal (Irkutsk)]. — 2014. — T. 127. — ‹ 4. — P. 48–52.
37. Garson C. Lack of cardioprotection by single-dose magnesium prophylaxis on isoprenaline-induced myocardial infarction in adult Wistar rats / C. Garson, R. Kelly-Laubscher, D. Blackhurst, A. Gwanyanya // Cardiovascular journal of Africa. — 2015. — Vol. 26. — ‹ 6. — P. 242–249.
38. Mutiso S. Relationship between anthropometric measures and early electrocardiographic changes in obese rats / S. Mutiso, D. Rono, F. Bukachi // BMC Research Notes. 2014. — Vol. 931. — ‹ 7. — P. 126–135.
39. Gui L. Ventricular tachyarrhythmias in rats with acute myocardial infarction involves activation of smallconductance Ca2+-activated K+ channels / L. Gui, Z. Bao, Y. Jia, X. Qin, Z. Cheng, J. Zhu, Q. Chen // Am J Physiol Heart Circ Physiol. — 2013. — ‹ 304. — P. 118–130.
40. Farraj A. The utility of the small rodent electrocardiogram in toxicology / A. Farraj, M. Hazari, W. Cascio // Toxicol Sci. — 2011. — Vol. 121. — ‹ 1. — P. 11–30.
41. Chrastina A. Experimental model of transthoracic, vascular-targeted, photodynamically induced myocardial infarction / A. Chrastina, P. Pokreisz, J. Schnitzer // Am J Physiol Heart Circ Physiol. — 2014. — ‹ 306. — P. 270–278.
42. Baillard C. Converting enzyme inhibition normalizes QT interval in spontaneously hypertensive rats / C. Baillard, P. Mansier, P. V. Ennezat, L. Mangin, C. Medigue, B. Swynghedauw, B. Chevalier // Hypertension. –2000. — Vol. 36. — ‹ 3. P. 350–354.
43. Lionte C., Bologa C., Sorodoc L. Toxic and DrugInduced Changes of the Electrocardiogram. In: Millis RM, editors. Advances in Electrocardiograms-Clinical Applications. Shanghai: Intech. — 2012. — P. 271–296.
44. Jiménez-Candil J. QT interval and acute myocardial ischemia: past promises, new evidences / J. JiménezCandil, C. Martín-Luengo // Rev Esp Cardiol. — 2008. — Vol. 61. — P. 561–563.
45. Braam S. Prediction of druginduced cardiotoxicity using human embryonic stem cell-derived cardiomyocytes / S. Braam, L. Tertoolen, A. Stolpe, T. Meyer, R. Passier, C. Mummery // Stem Cell Res. — 2010. — Vol. 4. — P. 107–116.
46. KrÛl M. Characterization of Disopyramide derivative ADD424042 as a non-cardiotoxic neuronal sodium channel blocker with broad-spectrum anticonvulsant activity in rodent seizure models / M. KrÛl, M. Ufnal, B. Szulczyk, P. Podsadni, A. Drapała, J. Turło, M. Dawidowski // Eur J Pharm Sci. — 2016. — ‹ 81. — P. 42–51.
47. Funck-Brentano C. Rate-corrected QT interval: techniques and limitations / C. Funck-Brentano, P. Jaillon // Am J Cardiol. — 1993. — Vol. 72. — ‹6. — P. 17–22.
48. Kmecova J. Heart rate correction of the QT duration in rats / J. Kmecova, J. Klimas // Eur J Pharmacol. — 2010. — Vol. 641. — ‹ 2–3. — P. 187–92.
49. Paronik V. Ä. Influence of cardioprotectors on the protease-inhibitory potential of the blood and heart of rats with adrenaline-induced myocardial ischemia / V. Ä. Paronik // Bulletin of Dnipropetrovsk University. — 2016. — ‹ 7 (1). — P. 27–31.
50. Tavares J. Epilepsy-induced electrocardiographic alterations following cardiac ischemia and reperfusion in rats / J. Tavares, E. Vasques, R. Arida, E. Cavalheiro, F. Cabral, L. Torres, F. Menezes-Rodrigues, A. Jurkiewicz, A. Caricati-Neto, C. Godoy, S. Gomes // Brazilian Journal of Medical and Biological Research. — 2015. — Vol. 48. — ‹ 2. — P. 140–145.
51. Schetinin P. P. Antiarrhythmic activity of dibornol in the conditions of the model of acute ischemia of myocardial reperfusion / P. P. Schetinin // Bulletin of Siberian medicine. — 2013. — V. 12. — ‹ 3. — P. 153–156.
52. Ryakhovsky A.E. Changes in the blood gas composition of rats in acute carbon monoxide poisoning / A.E. Ryakhovsky, D. A. Enikeev, G. R. Galyautdinova, L. E. Efimova, A. A. Yumadilova // Innovative Science. — 2017. — ‹ 10. — P. 88–91.
53. Smeyanova L. Ä. Dynamics of ECG with total thyroidectomy in rats / L. A. Smeyanova, A. H. Kade, S. A. Zanin, K. A. Lieva // International Journal of Experimental Education. — 2013. — ‹ 11–1. — P. 122–123
Review
For citations:
Privalova I., Shevelev O., Hodorovich N., Kuznetsova T., Glotova I., Legostaeva T., Ozerova I. Electrocardiography in rats in experimental studies (review of the literature). Genetics and breeding of animals. 2019;(2):108-120. (In Russ.) https://doi.org/10.31043/2410-2733-2019-2-108-120
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