Influence of DGAT1 and GH genes on milk productivity of red belarusian cow group

Abstract

The work presents the results of studies on the influence of diacylglycerol O-acyl transferase 1 (DGAT1) and somatotrophin (GH) genes taking into account their polymorphism on the milk productivity of red Belarusian cows. The object of the research was biological material from cows of red Belarusian breed contained in the USP "Novi Dvor-Agro" of the Svislochsky district of the Grodno region. DNA diagnostics of genotypes by the genes diacylglycerol O-acyl transferase 1 (DGAT1) and somatotrophin (GH) were carried out using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). As a result of the research carried out on the population of cows of the red Belarusian breed, the polyforphism of the gene somatotrophin (GH), represented by two alleles - GH^L and GH^V, with three genotypes GH^LL, GH^LV and GH^VV identified. Among the test animals, individuals with a GH^LL allele variant of 66% were more likely to cows, the GH^LV was identified in 32%, and the GH^VV was detected in only 2% of cows. By the gene diacylglycerol O-acyl transferase 1 (DGAT 1) polymorphism was identification, all animals had only one genotype, DGAT1^KK. Results of studies conducted on the effects of diacylglycerol O-acyl transferase 1 genes (DGAT1) and somatotrophin (GH) on milk productivity indices of cows of the red Belarusian breed group showed that in most cases animals with genotype GH^LL outnumbered their peers GH^LV and GH^LL, in terms of 305 days of lactation per 0.9-10.1%, fat and protein flakiness per 0.2-0.3 p.p., the amount of milk fat and protein in milk per 1.1-9.1%. Evaluation of milk productivity of cows by the gene diacylglycerol O-acyl transferase 1 (DGAT 1) in dynamics shows that with increase of lactation sequence number milk productivity indices increase.