Comparative characteristics of the genetic diversity in groups of trout of the rostal breed by multilocus analysis
https://doi.org/10.31043/2410-2733-2020-2-44-49
Abstract
Aquaculture provides the population with high-quality food products containing a number of valuable vitamins, minerals and omega acids. Breeding fish in artificial conditions is difficult without creating new and productive fish breeds. The species of rainbow trout turned out to be in steady demand in the market. The Rostal breed, originated from the SGC “Ropsha” through individual and family breeding, is highly adaptable and productive in cold-water conditions. The aim of the work was to identify the features of the genetic structure of various groups of rainbow trout of the Rostal breed by multilocus analysis using a digoxygenin-labeled DNA probe (GGAT)4. Using methods to simultaneously detect multiple polymorphisms increases the analysis throughput. Modern computer programs based on the distribution of DNA fragments calculate population genetic parameters. Earlier, we carried out work on rainbow trout with revealing the features of genetic organization at the level of populations of different breeds. In the framework of this study, the characteristics of individual groups of trout from various basins and having a different origin were revealed. It was established that individual groups have marker DNA fragments that distinguish them from other groups. The maximum genetic divergence was found between group A and group C, group B and group D. The groups A and B, B and C were moderately distant from each other. The groups D and C, A and D turned out to be closest in our study. The data helps breeders to take a scientifically sound approach to the breeding process by selecting individuals or groups of individuals with known genetic characteristics. Studies have shown that with long-term breeding of the group by family, a decrease in heterozygosity is observed, however, with the transition to mass selection, genetic diversity increases.
About the Authors
V. Terletskiy
Russian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Science Center for Animal Husbandry
Russian Federation
Russian Federation
Dr. Habil. (Biol. Sci)
196601, St. Petersburg, Pushkin, Russia, Tyarlevo, Moscovskoe sh. 55a
V. Tyshchenko
Russian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Science Center for Animal Husbandry
Russian Federation
Russian Federation
PhD (Biol. Sci)
196601, St. Petersburg, Pushkin, Russia, Tyarlevo, Moscovskoe sh. 55a
Yu. Scherbakov
Russian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Science Center for Animal Husbandry
Russian Federation
Russian Federation
Post graduate student
196601, St. Petersburg, Pushkin, Russia, Tyarlevo, Moscovskoe sh. 55a
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Review
For citations:
Terletskiy V., Tyshchenko V., Scherbakov Yu. Comparative characteristics of the genetic diversity in groups of trout of the rostal breed by multilocus analysis. Genetics and breeding of animals. 2020;(2):44-49. (In Russ.) https://doi.org/10.31043/2410-2733-2020-2-44-49
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