Study of criteria for selection and evaluation of donor cows in their preparation for embryo transplantation

Abstract

In the present studies, we studied and tested various criteria for evaluating donors before carrying out fetal transplantation. The main objectives of the study were: to study the prevalence of gynecological pathology in cows, which are potential candidates for embryo transplantation, to determine the relationship between the concentration of carotene and the level of progesterone as a marker of ovarian activity. It was found that the greatest number of complications during childbirth were recorded during the period of fetal excretion, which were observed in 72% of animals, and in the subsequent stage of labor, which were observed in 68% of animals. In the postpartum period, inflammation of the genital organs was most often manifested in 23.6% and uterine subinvolution 13.89%. Further, in the postpartum period, the concentration of progesterone in milk in cows was investigated and the functional activity of the corpus luteum was determined. For the study, a group of 32 cows was formed whose genital involution had ended. The study of progesterone was carried out in dynamics, twice on the 35th and 45th days after calving. The study found that in 71.86% of cows the amount of progesterone was less than 5 ng / ml, which indicates a low activity of the corpus luteum. In 28.13% of animals the concentration of progesterone on the 35th day was higher than 5.67 ng / ml and averaged 8.22±3.83 ng / ml and significantly decreased to 1.86 ± 1.69 ng / ml. The number of cows with a consistently low P4 concentration in two measurements was 56.25% of the total number of animals in the group. Thus, it was found 12.5% with a reduced activity of the corpus luteum in the luteal phase. Also, in experimental animals, a biochemical study of blood serum was carried out and a pronounced relationship with the concentration of carotene in the blood was determined.