Phytocorrection of biochemical status and productivity when fattening barrows on low-protein diets enriched with essential amino acids

The effect of the plant ecdysteroid-containing preparation of Leuzea extract on the metabolism and productivity of barrows (three-breed crossbreeds Landrace × large white × Duroc) during fattening on low-protein diets enriched with essential amino acids from 130 to 212 days old was studied for the first time. In the control and experimental groups, a low-protein barley-wheat diet with the addition of essential amino acids contained (g / kg) crude protein - 108 g, metabolic energy - 12.5 MJ, lysine - 7.6 g, threonine - 5.4 g, methionine - 5.2 g. In the experimental group, liquid extract of leuzea was introduced into the feed, at the rate of 190 mg / kg. Feeding the barrows during the fattening period of the leuzea extract contributed to an increase in the efficiency of the use of nutrients in biosynthetic processes in animals. In the barrows of the experimental group, the concentrations of total protein (P≤0.05), albumin (P≤0.05), creatinine (P≤0.05), lactate dehydrogenase, creatine kinase, alkaline phosphatase activity at a lower level were statistically significantly higher urea (16.1%, P≤0.05) and glucose (P≤0.05). In the experimental group, during the period of fattening, the average daily gain in live weight was higher (by 10.2%, P≤0.05), the yield in the carcass of muscle tissue (P≤0.05), the indices of lean and meatiness, and lower the cost of feed (P≤ 0.05), crude protein (P≤0.05) and exchange energy (P≤0.05) per 1 kg of gain in live weight, output of subcutaneous adipose tissue and internal fat in comparison with the control. It was concluded that the use of a leuzea extract containing phytoecdysteroids, while feeding low-protein diets enriched with lysine, methionine and threonine, corrects metabolic processes, stimulating the biosynthesis of meat components, and thereby contributes to an increase in the productivity of barrows during fattening.

phytoecdysteroids, leuzea extract, boars, fattening, low-protein diets, essential amino acids, phytocorrection of metabolism, biochemical parameters, productivity

1. Volodin V.A., Sidorova Yu.S., Maso V.K. 20-hydroxyecdysone - a plant adaptogen: anabolic effect, possible use in sports nutrition // Nutrition Issues - 2013 - T. 82 - No. 6 - P. 24-30.

2. Gumenyuk G.A., Cherkasskaya N.V. Guidelines for the study of feed and animal products. - Kiev: Urajay, 1977. - 256 p.

3. Erimbetov K.T., Obvintseva O.V. Modern approaches to rationing the nutrition of young pigs / Scientific fundamentals of livestock: sb.n. tr / GNU VNIIZh Agricultural Academy. - Dubrovitsy: VNIIZh. - 2009. - Vol. 65. - P. 173-175.

4. Erimbetov K.T., Obvintseva OV, Mikhailov VV Metabolism of nitrogenous substances and the formation of productivity in young pigs raised on low protein diets with different levels and ratios of essential amino acids // Problems of the biology of productive animals. - 2011. - No. 3. - P. 64-71.

5. Erimbetov K.T., Obvintseva O.V., Rodionova O.N. Improving the nutrition system of young pigs // Fundamental and applied aspects of feeding farm animals: Materials of the international. scientific-practical conference dedicated to the 100th anniversary of the birth of A.P. Kalashnikov / FSBI VIZH them. L.K. Ernst, comp.: R.V. Nekrasov, E.N. Delyagina, S.A. Nikitin.-Dubrovitsy: FSBI Federal Research Center VIZH them. L.K. Ernst, June 13-16, 2018.- P. 96-98.

6. Erimbetov K.T., Obvintseva O.V., Solovyova A.G. The effect of the addition of 20-hydroxyecdysone on the metabolism of nitrogen metabolism and productivity of piglets during the period of intensive cultivation // Problems of Biology of Productive Animals. - 2019.- No. 4. - P. 44-52. DOI: 10.25687 / 1996-6733.prodanimbiol.2019.4.44-52.

7. Zhavoronkov L.P. Fundamentals of applied biomedical statistics. Toolkit. Obninsk: Federal State Budgetary Institution MRRC of the Ministry of Health and Social Development of Russia. - 2012.- 60 p.

8. 8. Lebedev P.T., Usovich A.T. Research methods for animal feed and tissue. - M.: Rosselkhozizdat, 1976.- 389 p.

9. Nasyrov S.S., Khushbaktova Z.A., Syrov V.N. The results of an experimental study of phytoecdysteroids as stimulators of erythropoiesis in laboratory animals // Experiment. The Clinic. Pharmacol. - 1997. −№ 3. - P. 41-45.

10. Rosly I.M., Vodolazhskaya M.G. Comparative approaches in assessing the state of humans and animals: 1. cytolytic syndrome or fundamental mechanism? // Bulletin of Veterinary Medicine. - 2007. - No. 43. - P.63-66.

11. Anthony T.G. Mechanisms of protein balance in skeletal muscle // Domest. Anim. Endocrinol. − 2016. – Vol. 56. – P. S23–S32. 10.1016/j.domaniend.2016.02.012.

12. Anthony T.G., Mirek E.T., Bargoud A.R., Phillipson-Weiner L, DeOliveira C.M., Wetstein B., Graf B.L., Kuhn P.E., Raskin I. Evaluating the effect of 20-hydroxyecdysone (20HE) on mechanistic target of rapamycin complex 1 (mTORC1) signaling in the skeletal muscle and liver of rats // Appl. Physiol. Nutr. Metabol. – 2015. – Vol. 40. – P. 1324-1328.

13. Erimbetov K.T., Obvintseva O.V., Fedorova A.V., Zemlyanoy R.A., Solovieva A.G. Phenotypic regulation of animal skeletal muscle protein metabolism // Ukrainian Journal of Ecology. – 2019. - Т. 9. - № 4. – С. 651-656.

14. Folch J., Lees M., Sloane-Stanley G. H. A sample method of the isolation and purification of total lipids from animal tissue // J. of Biol. Chem. 1957. P. 497 − 509.

15. Li Y.H., Li F.N., Duan Y.H., Guo Q.P., Wen C.Y., Wang W.L., Huang X.G., Yin Y.L. Low-protein diet improves meat quality of growing and finishing pigs through changing lipid metabolism, fiber characteristics, and free amino acid profile of the muscle//J. Anim. Sci.- 2018- Vol.96 (8) – P. 3221-3232.

16. Yoshida T. Otaka, M. Ushiyama. Effect of ecdysterone on hyperglycemia in experimental animals // Biochem. Pharmacol. − 1971. − Vol. 20. − P. 3263-3268.