Cryopreservation of semen of initial forms and chimeras of birds of two breeds: Sussex light and Poltava clay

Purpose: evaluation of the quality of seed chimer and source forms before and after cryopreservation.

Materials and methods. For research, the breed of Sussex chickens and Poltava clay. Using these rocks, chimeras were obtained by the method of transplantation of blastodermal cells. An assessment of the quality of sperm of the roosters was carried out under a microscope at a temperature of 42 degrees. At various times, the activity of native sperm and its concentration was determined. Cryoconservation was carried out in small granules. The defrosting of the granules was produced on a heated metal plate at a temperature of 60 ° C.

Results. A comparative analysis of the volume of the ejaculate showed that Himer on average it was somewhat larger than that of the initial forms. The activity of sperms in native genital cells was sufficiently high in all studied samples, and was 80% or more. The concentration of sperm retained the same trend and practically did not differ from each other in all studied samples. The activity of frozen-fatty seed of the initial breeds and their chimer was significantly different. The maximum activity was observed in the Sussex breed and reliably below (p≤ 0.01) it has the remaining experimental groups. The activity of frozen-fatty seed turned out to be equal in chimeric organisms and Poltava clay, although Himer recipients were sussexes. It should be noted that the swing of oscillations according to the degree of activity of frozen-fatty seed in chimeric birds was significant (from 10 to 40 points), and the number of observations is small.

Conclusion. The study assess the quality of seed chimer and the source forms before and after cryopreservation. It was demonstrated that after defrosting the activity of frozen-fatty seed of the initial rocks and their chimer was different.

1. Fisinin V. I. Cryopreservation of male germ cells as a method of preserving the genetic resources of agricultural poultry / V. I. Fisinin, V. A. Bagirov, N. A. Volkova, N. A. Zinovieva, J. S. Roiter / / Achievements of science and agro-industrial complex technology. – 2012. – V. 8.

2. Tae H. J. Cryopreservation and its clinical applications / H. J. Tae, C. P. Sung, H. Y. Ji, Y. K. Jung, H. S. Jae, S. P. Ui, W. C. Chang, R. L. Sung, H. Jin // Integr. Med. Res. – 2017. – V. 6 (1). – P. 12-18.

3. Woelders H. Animal genetic resources conservation in the NNetherlands and Europe: poultry perspective / H. Woelders, C. A. Zuidberg, S. J. Hiemstra // Poultry Sci. – 2006. – V. 85. – P. 216-222.

4. Purdy P. H. Evaluation of glycerol removal techniques, cryoprotectants, and insemination methods for cryopreserving rooster sperm with implications of regeneration of breed or line or both / P. H. Purdy, Y. Song, F. G. Silversides and H. D. Blackburn // Poultry Sci. – 2009. – V. 88. – P. 2184-2191.

5. Santiago-Moreno J. Semen cryopreservation for the creation of a Spanish poultry breeds cryobank: optimization of freezing rate and equilibration time / J. Santiago-Moreno, C. Castano, A. Toledano-Diaz, Lopez-Sebastian, M. T. Prieto, J. L. Campo // Poultry Sci. – 2011. – V. 90. – № 9. – P. 2047-2053.

6. Narubina L.E. Method of cryopreservation of cock semen in the form of granules / L. E. Narubina, A. D. Kurbatov, G. B. Bublyaeva, K. V. Tselyutin / / USSR author's certificate No. 1343587, 1987.

7. Kozikova L.V. Chimeras of birds: methods of obtaining and prospects for their use. / L. V. Kozikova // Poultry. – 2019. – Vol. 10. – №9. – P. 9-13.

8. Tselyutin K. V. Cryopreservation of bird sperm as a tool for preserving the gene pool / K. V. Tselyutin, B. K. Tur // Genetics and breeding of animals. – 2009. – V.1. – P. 50-52.

9. Nauk V. A. An improved method of long-term preservation of the semen of bulls of producers in deep frozen state / V. A. Nauk / / Improvement of methods of reproduction of farm animals. – Chisinau, 1979. – P. 3-37

10. Fujihara N. Simple and rapid cryopreservation of rooster spermatozoa / N. Fujihara, S. Ohboshi // Low Temperature Medicine. – 1991. – V.17. – P. 12.

11. Van Voorst A. Fertility rate of daily collected and cryopreserved fowl semen / A. Van Voorst, F. R. Leenstra // Poultry Sci. – 1995. – V.74. – P. 136-140.

12. Wishart G. J. Quantitation of the fertilizing ability of fresh compared with frozen and thawed fowl spermatozoa / G. J. Wishart // Br. Poultry Sci. – 1985. – V.26. – P. 375-380.