Natural bee honey and Conservation of drone semen in liquid nitrogen
https://doi.org/10.31043/2410-2733-2021-4-17-22
Abstract
Purpose: Conducting a honey diluent test for creeples of sperm of a drone honey bee.
Materials and methods. The material for the research served a sperm of the milled drone drums of the "Prioksky" type of the Midway breed of bees. The selection of sperm was carried out in June-July 2020 g by the method of artificial stimulation of the turning of the endofalosha in half-armed drones aged 25-30 days. The rock type "Prioksky" of the middle Russian breed of bees. Before freezing, the sperm was stored in glass capillaries in the cooled state at 3 ° C for 2 months. The following composition of the diluent was tested - 10% honey, lactose, sucrose, egg yolk and dimethyl sulfoxide.
Results. Studies have shown the viability of sperm at 64.0 ± 1.8% (41.5-83.7), and a total mobility of 2.2 ± 0.6% (0-11.5). To evaluate the fertilizing ability of sperm, carried out artificial insemination of 10 bee modules. In 4 seeded bees dykens, the presence of sperm in a seed-hearter with a concentration of sperm from 0.22-4.4 million / μl is revealed. In paired eggs of three other seeded matters, the presence of sperm and the complete absence of spermatozoa in the seed-receptionist are recorded.
Conclusion. Tests of the honey diluent for deep freezing sperm of the drone honey bees in liquid nitrogen confirmed its cryophylactic properties.
About the Authors
A. Gulov
Federal state budgetary scientific institution “Federal beekeeping research centre”
Russian Federation
Russian Federation
researcher
391110, Ryazan region, Rybnoe, Pochtovayа st., 22
A. Laskin
Federal state budgetary scientific institution “Federal beekeeping research centre”
Russian Federation
Russian Federation
junior researcher
391110, Ryazan region, Rybnoe, Pochtovayа st., 22
References
1. Gulov A. N. Influence of synthetic media, egg yolk and bees honey on the cryptocrecy of sperm drutones / A. N. Gulov, Z. N. Saifutdinova, D. V. Mitrofanov, I. A. Languages [Electronic resource] // Genetics and Breeding animals. – 2020. – №1. – P. 27-36 Access mode: https://doi.org/10.31043/2410-2733-2019-4-3-8
2. Gulov A. N. Honey Diluent for the cryopreservation of sperm of the drone honey bee / A. N. Gulov, A. S. Laskin [Electronic resource] // Genetics and breeding of animals. – 2020. – №4. – P. 50-55 Access mode: https://doi.org/10.31043/2410-2733-2020-4-48-53
3. Hopkins B. K. Sequential generations of honey bee (Apis mellifera) queens produced using cryopreserved semen / B.K. Hopkins, C. Herr, W.S. Sheppard // Reproduction, Fertility and Development. – 2012. – V. 24. - Р. 1079-1083.
4. Gulov A. N. Problems of maintaining genetic resources of a honeybee / A. N. Gulov // Beekeeping. – 2018. – №6. – P. 22-25.
5. Gulov, A.N. Prospects for short-term storage of sperm drone honey bees / A. N. Gulov, E. O. Lagkina // Genetics and breeding of animals. – 2018. – №4. – P. 61-66.
6. Pinaev G.P. Methods of cell cultivation / G.P. Pinaev, M.S. Bogdanova // Polytechnic Publishing House. University, St. Petersburg, 2008. – 278 p.
7. Bieńkowska, М. Influence of the age of honey bee queens and dose of semen on condition of instrumentally inseminated queens kept in cages with 25 worker bees in the colonies [Электронный ресурс] /M. Bieńkowska, P. Węgrzynowicz, B. Panasiuk, K. Loc // Journal of Apicultural Science. – 2008. – V. 52. – № 2. – Р. 23-33.
8. Ruttner, F. Instrumental insemination of bee modules / F. Ruttner. – Bucharest: Apimonland, 1975. – 127 p.
9. Rhodes J. W. Semen production in drone honeybees. RIRDC Pub. / J. W. Rhodes // 2008. – No. 08/130. [Electronic resource].
10. Vasiliadi G. K. Srimathek – indicator of the quality of bee modules / G. K. Vasiliadi // Agricultural biology (animal biology series). – 1991. – № 6. – P. 76-81.
11. Brovarsky V. D. Migration of sperm and the development of ovaries of bee uterus / V. D. Brovarsky // Beekeeping. – 2005. – № 6. – P. 22-25.
12. Cobey S. W. Comparison studies of instrumentally inseminated and naturally mated honey bee queens and factors affecting their performance [Electronic resource] // Apidologie. – 2007. – V. 38. – Р. 390-410. - Режим доступа: https://hal.archives-ouvertes.fr/hal-00892280/document.
13. Woyke J. Influence of the number of attendant workers on the results of instrumental insemination of honey bee queens kept at room temperature [Электронный ресурс] / J. Woyke, Z. Jasinski // Apidologie. – 1980. – V. 11. – Р. 173–179. - Режим доступа: https://www.apidologie.org/articles/apido/.
14. Woyke, J. Influence of the number of attendant workers on the number of spermatozoa entering the spermatheca of instrumentally inseminated queens kept outdoors in mating nuclei [Электронный ресурс] / J. Woyke, Z. Jasinski // J. Apicultural Science, 1982. – V. 21. – Р. 129-133.
15. Mannapov A. G. Assessment of technologies of instrumental insemination / A. G. Mannapov, V. V. Lyakhov, V. D. Brovarsky // Beekeeping. – 2013. – №6. – p. 21.
Review
For citations:
Gulov A., Laskin A. Natural bee honey and Conservation of drone semen in liquid nitrogen. Genetics and breeding of animals. 2021;(4):17-22. (In Russ.) https://doi.org/10.31043/2410-2733-2021-4-17-22
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