Prognostic value of some leukocyte indices of intoxication and reactivity in fish under the action of physico-chemical stressors

Purpose: determining the prognostic value of leukocyte indexes of intoxication and reactivity in fish under the action of some physicochemical stressors.

Materials and methods. The object of the study was the lady of the roach of ordinary Rutilus Rutilus (L.). Control and experimental groups were formed taking into account the principle of analogues (n = 90). The average mass (PP) is 5.29 ± 1.0 g, length (TL) - 7.22 ± 0.39 cm, fish of the juvenile period of development (the sexual structure was not installed). The individuals were accumulated (10 days) and contained the following indices in aquariums (volume - 200 l.) We examined the core index, a leukocytic index of intoxication, blood leukocyte shear index, and a leukocytal index. As physico-chemical stressors, subcutal concentrations of copper (exposition in copper salt 0.01 mg/l and 0.1 mg/l for 7 and 14 days) were studied; imitation of the main phase and the initial period of the phase of geomagnetic storm; The action of high temperature.

Results. The analysis of leukocyte indexes of intoxication and reactivity showed that in fish the Krebs index and a leukocytic index have the greatest prognostic importance. The information content of the blood leukocyte shear index and the leukocyte intoxication index should be considered controversial. The blood leukocyte shift index increased only with the exposure of copper salts for 14 days and with the action of physical stressors. Statistically significant changes in the blood leukocyte shift index during exposure by copper salts for 7 days have not been revealed, which may indicate a low sensitivity of the index. The results obtained during the calculation of the leukocyte index of intoxication are similar to the results when determining the Krebs index. Specific changes in the index under the action of physico-chemical stressors have not been identified.

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