Immunological status of the blood of clinically healthy and lactating cows with mastitis
https://doi.org/10.31043/2410-2733-2023-4-59-63
Abstract
Mastitis in high yielding lactating cows is the most common disease that causes enormous economic damage to livestock farms. According to leading scientists, when studying the problem of mastitis, the greatest interest is in studying the dynamics of changes in the immunological blood indicators of cows during the occurrence and development of the inflammatory process in the udder. To conduct research, three groups of lactating Holstein cows of 2–3 lactations with an annual milk production of 7356–8000 kg of milk, belonging to one of the farms in Voronezh region, were formed. The first group (n=8) included clinically healthy animals, the second (n=8) - cows with subclinical mastitis, the third (n=8) - cows with clinically pronounced catarrhal mastitis. Blood samples were taken from all animals to determine immunobiochemical and molecular genetic indicators. The studies have established that the level of expression of the genes for the pro- and anti-inflammatory cytokines IL-10 and IL-4 was by 2.6 and 1.5 times lower, respectively, in the cows with subclinical mastitis and by 5.8 and 3.9 times lower in the cows with clinically pronounced catarrhal mastitis, respectively, in comparison with clinically healthy animals. At the same time, the expression of pro-inflammatory INF-γ was by 7.6 and 14.8 times higher in the cows with subclinical and catarrhal mastitis, respectively, compared to clinically healthy animals.
About the Authors
V. Zimnikov
Federal State Budgetary Institution "All-Russian Research Veterinary Institute of Pathology, Pharmacology and Therapy"
Russian Federation
Russian Federation
PhD (Vet. Sci.)
394087, Voronezh, st. Lomonosova, 114b
L. Sashnina
Federal State Budgetary Institution "All-Russian Research Veterinary Institute of Pathology, Pharmacology and Therapy"
Russian Federation
Russian Federation
Dr. Habil. (Vet. Sci.)
394087, Voronezh, st. Lomonosova, 114b
N. Pasko
Federal State Budgetary Institution "All-Russian Research Veterinary Institute of Pathology, Pharmacology and Therapy"
Russian Federation
Russian Federation
PhD (Biol. Sci.)
394087, Voronezh, st. Lomonosova, 114b
References
1. Kamyshanov A. S. Study of biochemical and morphological parameters of the blood of cows during different periods of lactation with mastitis / A. S. Kamyshanov // International Scientific Research Journal. – 2021. – № 3(105). – P. 48-52. DOI: 10.23670/IRJ.2021.105.3.033.
2. Antsiferova Yu. S. Cytokines in obstetrics, gynecology and neonatology / Yu. S. Antsiferova, N. Yu. Sotnikova, M. N. Shokhina // Cytokines and inflammation. – 2002. – № 2(1). – Р. 145-156.
3. Zhidovinov A. A. Clinical and laboratory stratification of endogenous intoxication and SIRS in patients with a common form of appendiceal peritonitis / A. A. Zhidovinov, S. V. Chuprov // Cytokines and inflammation. – 2007. – № 6(1). – P. 25-30.
4. Simbirtsev A. S. Cytokines: classification and biological functions / A. S. Simbirtsev // Cytokines and inflammation. – 2004. – № 3(2). – P. 16-22.
5. Serebrennikova S.N. Interleukin-1, interleukin-10 in the regulation of the inflammatory process / S.N. Serebrennikova, I.Zh. Seminsky, N.V. Semenov, E.V. Guzovskaya // Siberian Medical Journal. – 2012. – № 8. – P. 5-7.
6. Chubenko V. A. Immunotherapy is based on cytokines (IL-1, IL-2, FNO, CSF, IFN) / V. A. Chubenko // Hum.Reprod. – 2016. – № 5. – P. 35-41.
7. Yazd A. S. The interleukin-1 family / A. S. Yazd, K. Ghoreschi // Adv. Exp. Med. Biol. – 2016. – № 941. – P. 21-29. DOI: 10.1007/978-94-024-0921-5.
8. Cicinelli E., Matteo M., Tinelliet R. Prevalence of chronic endometritis in repeated unexplained implantation failure and the IVF success rate after antibiotic therapy // Hum.Reprod. – 2015. – № 30(2). – P. 323-353. DOI: 10.1093/humrep/deu292.
9. Kocharyan V. D. Biochemical parameters of blood and milk during mastitis in Holstein cows / V. D. Kocharyan, K. A. Bakanova, M. A. Ushakov, Zh. Sh. Ushakova // Current problems of intensive development of animal husbandry. – 2022. – №. 25-1. – Р. 69-76.
10. Zhdanova I. N. The influence of the phytobacterial complex BCL on the morphobiochemical status of cows with clinical mastitis / I. N. Zhdanova // Bulletin of the Perm Scientific Center of the Ural Branch of the Russian Academy of Sciences. – 2018. – № 3. – P. 51-57. DOI 10.7242/1998-2097/2018.3.5.
11. Immunological aspects of the fight against cow mastitis / V. Slobodyanik, N. Klimov, L. Cheskidova, E. V. Zverev. – Voronezh: Istoki Publishing House, 2020. – 222 p. – ISBN 978-5-4473-0284-9.
12. Kamyshanov A. S. Hematological parameters of cows during pregnancy with subclinical mastitis and the development of birth and postpartum diseases in them / A. S. Kamyshanov // BIO. – 2021. – № 4(247). – P. 8-11.
13. Methodological recommendations for the assessment and correction of nonspecific resistance of animals // New research methods on problems of veterinary medicine / A. M. Smirnov [etc.]. – Moscow: Russian Academy of Agricultural Sciences, 2007. – Ch. III.: Research methods on problems of veterinary pathology in productive animals. – pp. 174-215.
Review
For citations:
Zimnikov V., Sashnina L., Pasko N. Immunological status of the blood of clinically healthy and lactating cows with mastitis. Genetics and breeding of animals. 2023;(4):59-63. (In Russ.) https://doi.org/10.31043/2410-2733-2023-4-59-63
Views:
366
Email this article 