Morphological study of the liver of rats after the use of the mineral feed additive based on chelate compounds

Purpose: a morphological study of the liver of white laboratory rats of the Wistar line supplemented with the mineral feed additive based on chelate compounds.
Materials and methods. The experiment was carried out on male white Wistar laboratory rats weighing 200-250 g. “Chelavit C” was used as a mineral feed additive. After three months, the animals were euthanized using inhaled drugs combined with an injection overdose of anesthetic. Morphological analysis was performed on macro- and micropreparations of the liver. For a general assessment of the histological organization of the liver, hematoxylin-eosin staining was used. Iron accumulation in the liver was assessed using Perls Prussian blue stain.
Results. The differences in some essential clinical and morphological parameters assessed on macroscopic specimens of the liver of animals from the experimental and control groups were insignificant, being within the acceptable physical and anatomical norm. Morphometric analysis performed on histological preparations of the liver did not reveal differences between the groups, and the parameters were within acceptable standards. The Perls Prussian blue stain showed that the iron content in the liver of animals in the experimental and control groups corresponds to normal values (from 1+ to 3+); therefore, the feed additive based on chelate compounds does not cause hemosiderosis, which can later develop into hemochromatosis.
Conclusion. The mineral feed additive based on chelate compounds replenishes lost microelements without having a toxic effect on the liver of rats. However, when administering this additive to animals, it is necessary to accurately calculate the dosage to avoid possible toxic effects

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