Age dynamics of the biochemical profile of mixed foxes of the red marble color type

The study of biochemical blood parameters in caged foxes plays an important role in ensuring their health and preventing diseases. Blood components within physiological limits can change their balance during disease, making the blood biochemical profile a key tool in diagnosis. The body's enzyme system primarily responds to changes in environmental conditions, even in the absence of obvious clinical signs. The main metabolic indicators were studied (using “High Technology” (USA) reagent kits): total protein, albumin, alanine aminotransyaerase (AlAT), aspartate aminotransferase (AsAT), urea, creatinine, cholesterol, α-amylase. It was established that these analytes in crossbred foxes of the red marbled color type were within the generally accepted limits of the physiological norm for foxes. A study of the dynamics of AlAT (alanine aminotransferase) and AsAT (aspartate aminotransferase) levels in female and male foxes revealed general patterns in their changes during growth and development. A decrease in the level of analytes by 4 months of age and an increase by 6 months, indicating more intense metabolic processes in the body of foxes, especially on the eve of the winter period, which may be associated with the accumulation of body weight to maintain energy in the cold months. In females and males, there is a significant decrease in the level of alkaline phosphatase with age, reaching a decrease in females of 30 % (p≤0,05) by 4 months of age and 56,54 % (p≤0,05) by 6 months of age and in males by 33,06 % (p≤0,05) by 4 months and 51,76 % (p≤0,05) by 6 months, respectively, relative to the level noted in 1,5 month old puppies. In females, there was a relative decrease in total protein of 24,8 % (p≤0,05) between the ages of 1,5 and 4 months, and then an increase of 4,7 % by 6 months. Males also show a similar trend, with total protein levels decreasing by 38,9 % (p≤0,05) by 4 months, and then concentrations returning to levels at 1,5 months of age, with a difference of 6,6 %. Thus, the dynamics of changes in the studied analytes in female and male foxes indicates not only the physiological development of the organism during growth, but also highlights adaptive changes, which may be important for understanding their metabolic processes and general health.

1. Berezina Yu. A. Biochemical picture of blood of adult arctic foxes of different sexes and colors / Yu. A. Berezina, M. A. Koshurnikova et al. // Perm Agrarian Bulletin. - 2015. – № 3. – P. 54–58.

2. Berezina Yu. A. Biochemical picture of blood of silver arctic fox depending on sex and age / Yu. A. Berezina, M. A. Koshurnikova et al. // Veterinary science. — 2015. – V. 4. — P. 50–52.

3. Ilyina E. D. Fur farming / E. D. Ilyina, A. D. Sobolev, T. M. Chekanov. – SPb.: Lan Publishing House, 2004. – 304 p.

4. Batoev Ts. Zh. Ecological significance of seasonal variability of biochemical parameters of blood of American burrowers and silver-black foxes / Ts. Zh. Batoev, S. Ye. Sanzhieva et al. // Bulletin of the Buryat State University. Biology, Geography. – 2013. – № 4. – P. 179–184.

5. Antonova E. P. Age and seasonal changes in antioxidant protection of muscle tissue and morphometric parameters of erythrocytes in the muskrat (Ondatra zibethicus) / E. P. Antonova, V. A. Ilyukha, et al. / / Journal of Evolutionary Biochemistry and Physiology. – 2020. – Vol. 56. – № 5. – P. 359–367.

6. Ilyukha V. A. Fur animals as a model in biomedical research / V. A. Ilyukha, A. G. Kizhina, et al. // Rabbit breeding and animal husbandry. — 2021. – № 6. — P. 5—16. doi: 10.52178/00234885_2021_6_5.

7. Balakirev N. A. Features of feeding fur animals in modern conditions / N. A. Balakirev // Feeding of farm animals and forage production. — 2014. — № 5. — P. 55—60.

8. Balkhanovskaya T. V. Dependence of biochemical blood parameters of silver-black foxes on the season of the year in the north of the Tyumen region / T. V. Balkhanovskaya // Fundamental research. — 2013. — № 4—5. — P. 1120—1123.

9. Shumilina N. N. Features of the quality of pubescence in colored forms of foxes (Vulpes vulpes) / N. N. Shumilina, T. M. Chekalova, M. V. Mitrofanova // Information Bulletin of VOGiS. – 2007. – Vol. 11. — № 1. — P. 131—138.

10. Shumilina N. N. Commercial properties of fox pelts of different breeds / N. N. Shumilina, M. V. Mitrofanova // Rabbit breeding and fur farming. – 2000. – № 5. – P. 12.

11. Berestov V. A. Clinical biochemistry of fur animals: reference. manual / V. A. Berestov. – Petrozavodsk: Karelia, 2005. – 168 p.

12. Tyutyunnik N. N. Biochemical testing as a method for assessing the physiological state of fur animals bred in industrial complexes / N. N. Tyutyunnik, L. K. Kozhevnikova // Agricultural biology. – 1996. — № 2. – P. 39–49.

13. Trapezov O. V. Domestication as the Earliest Intellectual Achievement of Humanity / O. V. Trapezov // Vavilov Journal of Genetics and Breeding. — 2013. — Vol. 17. — № 4/2. – P. 872–883.

14. Berestov V. A. Scientific Foundations of Animal Husbandry / edited by V. A. Berestov. – L.: Nauka, 1985. – 477 p.

15. Koldaeva E. M. Breeds of Fur Animals and Rabbits / E. M. Koldaeva, L. V. Milovanov, O. V. Trapezov. – M.: KolosS, 2003. – 240 p.

16. Pereldik N. Sh. Feeding fur animals / N. Sh. Pereldik, L. V. Milovanov, A. G. Erin: M. — Agropromizdat, 1987. – 334 p.

17. Ivanter E. V. Elementary biometrics / E. V. Ivanter, A. V. Korosov. – Petrozavodsk: PetrSU, 2005. 104 p.

18. Bożena N.-D. Chosen Blood biochemical parameters in free-living wild and farmed minks, foxes and raccoon dogs / N.-D. Bożena, J. Andrzej //Veterinarija ir Zootechnika. – 2015. – № 70(92). – P. 48–52.

19. Berezina Yu. A. Dynamics of biochemical parameters of blood of the red fox (Vulpes vulpes L.) in ontogenesis / Yu. A. Berezina, M. A. Koshurnikova, I. A. Domsky, O. Yu. Bespyatykh // Ippology and veterinary science. – 2020. – № 2 (36). – P. 177–181.

20. Rubio A. V. Hematology and serum biochemistry values оf culpeo foxes (Lycalopex culpaeus) from Central chile /A. V. Rubio, E. Hidalgo-Hermoso, C. Bonacic// Journal of Zoo and Wildlife Medicine — 2014. – № 45(3). – P. 589–593. doi: 10.1638/2013-0280R2.1.

21. Lescano J. Hematology and serum biochemistry of free-ranging and captive Sechuran foxes (Lycalopex sechurae) / J. Lescano, M. Quevedo, M. Villalobos // Vet Clin Pathol. – 2018. — № 47. – P. 29–37.

22. Rui P. Hematology and serum biochemistry values in adult racoon dogs and foxes in Changli farms of Hebei Province, China // P. Rui, Z. Ma, X. Zhang [et. al] // African Journal of Microbiology Research. – 2011. – Vol. 5(26). – P. 4667–4672. doi: 10.5897/AJMR11.932.

23. Segal A. N. Some ecological and physiological characteristics of the Arctic fox (Alopex lagopus) / A. N. Segal, T. V. Popovich // Zool. Zhurn. – 1976. – Vol. 55. – Issue. 5. — P. 741–754.

24. Campbell J. M.The biological stress of early weaned piglets / J. M. Campbell, J. D. Crenshaw, J. Polo // Journal of Animal Science and Biotechnology. – 2013. – Vol. 4. – P. 19.

25. Hall D. Nutritional influences on estrogen metabolism. Applied Nutritional Science Reports. 2001. Vol. 1.

26. Saraç F. Causes of high bone alkaline phosphatase / F. Saraç, F. Saygılı // Biotechnology and Biotechnological Equipment. – 2007. – Vol. 21. – № 2. – P. 194–197.

27. Lala V., Zubair M., Minter D.A. Liver function tests. [Updated 2023 Jul 30]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024–. Available at: https://www.ncbi.nlm.nih.gov/books/NBK482489.

28. Belinskaia D. A. Integrative role of albumin: evolutionary, biochemical and pathophysiological aspects / D. A. Belinskaia, P. A. Voronina, N. V. Goncharov // Journal of Evolutionary Biochemistry and Physiology. – 2021. – Vol. 57. – № 6. – P. 1419–1448. doi: 10.1134/S002209302106020X.29.

29. Mohiuddin S. S., Khattar D. Biochemistry, ammonia. [Updated 2023 Feb 20]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024. Available at: https://www.ncbi.nlm.nih.gov/books/NBK541039.

30. Taegtmeyer H. Creatine— a dispensable metabolite? / H. Taegtmeyer, J.S. Ingwall //Circulation Research. – 2013. – Vol. 112. – № 6. – P. 878–880. doi: 10.1161/CIRCRESAHA.113.300974.31.

31. Craig M., Yarrarapu S. N. S., Dimri M. Biochemistry, cholesterol. [Updated 2023 Aug 8]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024–. Available at: https://www.ncbi.nlm.nih.gov/books/NBK513326.