Dynamics of changes in the quantitative and functional parameters of leukocytes when mineral feed additives are introduced into the diet

Purpose: a comprehensive assessment of the effect of chelated forms of trace elements on quantitative and qualitative characteristics of leukocytes in combination with growth processes. Materials and methods. The experimental part was carried out on male laboratory rats. 2 homogeneous groups of 5 individuals were formed: experimental and control. The feed additive “Helamax C” was introduced to the main diet of the experimental group. The experiment was conducted in 3 stages. At each stage, body weight measurements were carried out, as well as blood sampling with subsequent examination of leukocytes (counting the absolute and relative number of leukocytes, building leukograms, as well as analysis of leukocyte indices). Blood was taken from the caudal vein. Leukocytes were counted according to the generally accepted method using the Goryaev camera. Leukograms were calculated at each stage of the experiment, after which leukocyte indices were calculated.

Results. At the end of the experiment, there was an increase in the total number of leukocytes from 5,95±0,12 G/l to 7,47± 0,32 G/l in the blood of rats against the background of the use of a mineral supplement. A decrease in the absolute number of rod-shaped neutrophils from 0,19±0,08 G/l to 0,11±0,04 G/l, with a simultaneous increase in the level of segmented neutrophils to 1,39±0,18 G/l. In the rats of the experimental group, the absolute number of lymphocytes in the blood increased from 4.94±0,07 G/l to 5,64±0,26 G/l. There was an increase in the neutrophil lymphocyte ratio index from 0,06±0,03 to 0,26±0,02 in individuals of the experimental group, as well as a decrease in the nuclear shift index from 0,32±0,09 to 0,08±0,03. The body weight of the experimental rats increased from 368,40±20,27 g to 534,40±13,74 g.

Conclusion. The introduction of mineral supplements into the diet helps to improve the quantitative and qualitative indicators of leukocytes, stimulating and regulating their formation, differentiation, functioning and apoptosis. In addition, minerals have a positive effect on the immune system, increasing the body's natural resistance. Together with the activation and regulation of metabolic processes, this leads to stimulation of body growth and an increase in body weight.

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