Correction of lipid metabolism disorders in rat heart tissues by plant antihyposants against the background of acute histotoxic hypoxia

Hypoxia is a widespread pathological process that manifests itself in insufficient oxygen supply to the tissues of the body and/or impaired cellular uptake. This condition triggers a complex of secondary nonspecific metabolic and functional disorders, as well as adaptation reactions. Regardless of the causes and conditions contributing to the development of hypoxia, it leads to failures in the processes of biological oxidation and energy metabolism. The aim of the study was to investigate the disorders of lipid metabolism in rat heart tissues and the effect of plant antihypoxants on them during modeling of acute histotoxic hypoxia.

Materials and methods. The studies were performed on white mongrel rats weighing 240-260 g. A model of histotoxic hypoxia was used. Lipoproteins in rat heart tissues were determined by electrophoretic method according to the standard technique using standard sets of chemical reagents from Lahema (Czech Republic). Phospholipid spectrum in rat heart tissues was determined by thin-layer chromatography using silicone plates of Silufol (Czech Republic).

Results. The results of the studies showed that against the background of acute histotoxic hypoxia lipid metabolism disorders developed, characterized by a decrease in the concentration of lecithin, cephalin, phosphatidylserine, cardiolepin and an increase in the concentration of sphingomyelin and lysolecithin, as well as a decrease in the total concentration of phospholipids in general. The use of antihypoxants leveled the negative effect of histotoxic hypoxia on lipid metabolism in rat heart tissues and the most pronounced positive effect was observed when using a mixture of extracts of medicinal raspberry and black currant in the ratio of 1:1.

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