The main blood parameters of sheep when a chromium-glutamic acid complex is introduced into the diet

Animal blood is one of the most important systems for assessing both the state of the organism as a whole and specific organs and tissues. Among all farm animals, rams are the most frequently used for model physiological and biochemical studies, especially for fistula experiments.

Objective: to assess blood parameters and physiological and biochemical status of Romanov rams (as model animals) before and after the use of an organic complex of chromium (III) with glutamic acid as a feed additive.

Material and methods. The balance experiment was conducted on Romanov rams (N = 3). The chromium complex with glutamic acid was added to the feed of the 1st and 2nd experimental groups in two doses (0.33 and 3.30 mg in terms of pure chromium). The duration of each experiment was 28 days. Before and after the experiment, blood was taken from the jugular vein for hematological and biochemical analysis.

Results. When using this complex, blood analysis of rams showed that homeostasis of its metabolites and formed elements is maintained at the normal level corresponding to clinically healthy animals. Significant changes in the content of urea in the blood: in the first experimental group - it increased more significantly (by 3.8%) than in the 2nd experimental group (by 2.5%) - relative to the control group, which is associated with increased metabolism of proteins and other nitrogen-containing substances in the blood, since urea is the final metabolite of protein metabolism in rams. This is generally a positive effect and is certainly associated with the inclusion of a chromium complex with glutamic acid in the diet of animals. Reliable differences were found for such indicators as the amount of hemoglobin and hematocrit (in the second experimental group it increased by 16.9% and 7.6%, respectively, compared to the control).

1. Romanov V. N. An effective complex of biologically active substances for increasing the productivity of ruminants / V. N. Romanov, N. V. Bogolyubova, V. A. Devyatkin // Vestnik of the Ryazan State Agrotechnological University named after P. A. Kostychev. – 2017. – № 4 (36). – P. 60–65.

2. Romanov V. N. A study of the physiological effect of a biologically active supplement based on a probiotic and shungite in sheep and growing bulls / V. N. Romanov, N. V. Bogolyubova, A. V. Mishurov, R. A. Rykov // Problems of Biology of Productive Animals. — 2019. – № 2. – P. 54–63. doi: 10.25687/1996-6733.prodanimbiol.2019.2.54-63.

3. Zaitsev S. Yu. Evaluation of the efficiency of chromium compounds as additives in pig feeding / S. Yu. Zaitsev, O. N. Sivkina, O. A. Voronina // Pig breeding. – 2024. – № 6. – P. 59–64. doi: 10.37925/0039-713X-2024-6-59-64.

4. Voronina O. A. Biological role of chromium: rationale, experience and prospects for its use in cattle / O. A. Voronina, O. N. Sivkina, S. Yu. Zaitsev // International Bulletin of Veterinary Medicine. – 2024. – № 2. – P. 212–228. doi: 10.52419/issn2072-2419.2024.2.212.

5. Karpenya M. M. Effect of the feed additive "Nanoplant chrome (K)" on the morphological and biochemical parameters of the blood of breeding bulls / M. M. Karpenya, T. N. Nogina [et al] // The role of veterinary science and education in modern society: to the 100th anniversary of the Vitebsk Order of the Badge of Honor State Academy of Veterinary Medicine: materials of the International scientific and practical conference (Vitebsk, November 4–5, 2024) / Vitebsk State Academy of Veterinary Medicine. – Vitebsk: VGAVM, 2024. – P. 200–203.

6. Uyanik F. The effects of dietary chromium supplementation on some blood parameters in sheep / F.Uyanik // Biological Trace Element Research. – 2001. – Vol. 84. – P. 93–101. doi: 10.1385/BTER:84:1-3:093.

7. Dominguez–Vara I. A. Effects of feeding selenium-yeast and chromium-yeast to finishing lambs on growth, carcass characteristics, and blood hormones and metabolites / I. A. Dominguez–Vara, S. S. Gonzalez–Muñoz, J. M.Pinos–Rodriguez, J. L. Borquez–Gastelum, R. Barcena–Gama, G. Mendoza–Martinez, L. L. Landois–Palencia // Animal Feed Science and Technology. – 2009. – Vol. 152. – № 1–2. – P. 42—49. doi: 10.1016/j.anifeedsci.2009.03.008.

8. Ward T. L. Interactive effects of dietary chromium tripicolinate and crude protein level in growing-finishing pigs provided inadequate and adequate pen space / T. L. Ward, L. L. Southern, T. D. Bidner // Journal of animal science. – 1997. – Vol. 75. – № 4. – P. 1001–1008. doi: 10.2527/1997.7541001x.

9. Ovsyannikov A. I. Basics of Experimental Animal Husbandry. – Kolos, 1976.

10. Sivkina O. N. Development of a Method for Obtaining a Chromium Complex with Glutamic Acid as a Feed Additive for Animals / O. N. Sivkina, A. A. Vikhrov, S. Yu. Zaitsev // Problems and Prospects of Commodity Chemistry and Traditional Medicine. Proceedings of the XI International Scientific and Practical Conference (Andijan, 2024, September 11–12).

11. Biochemical Parameters of Blood Serum of Various Animal Species. https://www.ld.ru/reviews/ilist-4422.html (accessed May 2, 2025).