Analysis of TNS2 gene polymorphism and its association with productivity traits in Rainbow Trout Rofor

The objective of this study was to analyze the A/G polymorphism at position 17:453356000 in the TNS2 gene, its association with phenotypic traits in the Rofor rainbow trout individuals, and to investigate the structure of the size and weight characteristics within the examined sample.

Materials and Methods. The study involved two-year-old female rainbow trout Rofor breed (n=200), which morphometric data were collected, including body weight, total length to the end of the caudal fin, length to the end of the scaled body, head length, maximum body height, and body thickness. Genotyping was performed using a test system developed in our laboratory. Data analysis were provided in the R programming language utilizing the GGally package, as well as complementary analysis in Python using tools from the scikit-learn library.

Results. Two hundred female broodstock rainbow trout of the Rofor breed were successfully genotyped for the A/G single nucleotide polymorphism at position 17:453356000 in the TNS2 gene. The vast majority of individuals were heterozygous AG carriers (n=161). The study demonstrated a high interdependence among the morphometric traits of rainbow trout, with the GG genotype being associated with morphometric characteristics indicative of a larger body conformation. This research broadens current knowledge of rainbow trout genetics. Moreover, a genotyping test system for the A/G polymorphism in the exon region of the TNS2 gene was developed, offering a valuable tool for marker-assisted selection programs in rainbow trout breeding.

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