Genetic variability in 3 populations of interbreed hybrids of gene pool chicken of Bioresource collection of RRIFAGB

Gene pool breeds of chickens around the world are considered as a source of valuable genes, which may be in demand in breeding programs when market conditions change and, accordingly, the requirements for the quality of poultry products will appear. Identification of genetic changes occurring when crossing such breeds is the subject of research, since these data can solve some aspects of the inheritance of valuable genotypes in poultry generations. In the laboratory of molecular genetics, using a number of modern techniques for many years, a gene pool poultry has been studied. This study is a continuation of such research. As a research object, two-breed hybrids of several gene pool breeds of the bioresource collection chicken were selected in the number of 45 heads, blood from under the porch vein was taken to vacuum tubes for subsequent DNA isolation. High-molecular genomic DNA was then analyzed by DNA fingerprinting with a labeled oligonucleotide probe to identify hypervariable regions in the chicken genome. The distribution of DNA fragments on nylon filters was studied by pairwise comparison in all samples with calculation of the number of common and different fragments. The data was recorded in a table and further calculations were made using the Gelstats ™ computer program. Knowing the genealogy of the original gene pool of hens, it was interesting to trace the inheritance of hypervariable regions of the genome when crossing these breeds in the next hybrid generation. Data on marker DNA fragments that are characteristic of individual populations are also presented. In addition, the results of intrapopulation genetic variability, expressed as the average heterozygosity, are presented. The probability of occurrence of identical genotypes in individuals in the population for all detected DNA fragments was extremely low, which indicates on high resolution of the method used.

gene pool chicken breeds, oligonucleotide probe, DNA-fingerprinting, genetic variability, heterozygosity

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