Life-time differential diagnostics of reindeer helminthiases

The freshly excreted feces of the reindeer introduced into the Leningrad Region from the Murmansk region (2 farms) were studied by the methods of flotation and sedimentation ovooscopy, as well as by methods of larvae- and helminthoscopy at the Department of Parasitology, St. Peresburg State Academy of Veterinary medicine in February 2018. The study of the preparations was carried out using light microscopes MBS-10 (LOMO) and Mikmed-6 (LOMO). To obtain pictures an optical-mechanical adapter and camera 5D Mark II (Canon) were used. The oval eggs were found using modified Darling’s method in samples from a farm in the south of the Leningrad Region. Their length was 0.045-0.050 mm and the width was 0.022-0.030 mm. The content of the eggs is granular, the shell is relatively thick, multilayered. Eggs are clearly bipolar-plugeed. Based on their morphology, eggs can be attributed to the genus Capillaria. In samples from a farm in the north of the Leningrad region, Weid’s method revealed mobile larvae, the tail ends of which were curved and had spines. The length of the larvae was about 0.350 mm with a width of about 0.015 mm. Based on their morphology, the larvae can be attributed to the family Protostrongylidae. The physiological characteristics of the larvae, epizootic and clinical data suggest that the causative agent is the helminth of the genus Elaphostrongylus. Life-time identification of nematodes requires the use of molecular biology, serology, allergology and other tools. The need for such clarification is both of practical activities (the strategy of treatment and prevention) and of fundamental parasitology. The question of morphological differentiation of nematodes of the family Protostrongylidae based on their larvae L1 requires further study.

Capillaria, Elaphostrongylus, Parelaphostrongylus, Rangifer tarandus, Capillaria, Elaphostrongylus, Parelaphostrongylus, diagnostic parasitology, morphological diagnosis, reindeer, Rangifer tarandus

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