The influence of breeding methods on the molecular genetic structure of budenny horse breed
https://doi.org/10.31043/2410-2733-2019-2-97-102
Abstract
Budenny horse breed was obtained as a result of the crossing of Don and local Black Sea mares with Thoroughbred stallions during 1920-1920. To improve the athletic qualities of Budenny horses Thoroughbred stallions are regularly used in breeding, which inevitably affects the breeding qualities of the received offspring. The task of the research was to study the genetic features of the polymorphism of microsatellite loci in Budennny horses, taking into account the origin and Thoroughbred horses’ blood propotion. Tested on 17 loci of microsatellites, horse DNA of the Budenny breed (n = 155) were subdivided into 5 groups, taking into account the type of selection and the proportion of blood. In the 5th experimental group horses with unregistered ancestors in the pedigree from the “Pobeda” farm were included. A comparative genetic-population analysis of different groups of horses was performed on a number of indices, including the level of polymorphism (Ae), the observed (Ho) and the expected (He) heterozygosity, as well as Wright's F-statistics indices using Microsoft Excel 2010. As a result of testing 127 alleles of STR loci were identified in Budenny breed that evidences a sufficient reserve of genetic diversity. The number of alleles in the studied loci varied from 3 to 13, the most polymorphic ones were the ASB17 (13), VHL20 (9) ANT4 (9) and LEX3 (9) loci. Horses of the all 5 groups differed both in the spectrum of alleles and in the degree of heterozygosity. The highest level of the main parameters (MNA, Ae, Ho and He) had horses of a consolidated origin and Thoroughbred blood proportion at the level of 5/8. The practiced crossing with Thoroughbred stallions led to an increase in the proportion of blood and the decrease in Ae polymorphism level from 3.803 to 3.588, the degree of heterozygosity lowed from 0.737 to 0.703. The results of phylogenetic analysis indicate that the four groups of Budenny horses form a common cluster, in the center of which are horses with a consolidated origin. Microsatellite markers used to control the origin of horses can be additionally used for genetic control of breeding processes in populations and breeds of horses.
About the Authors
N. Blohina
FSBSI «ARRI for Horsebreeding»
Russian Federation
Russian Federation
PhD. (Agr. Sci.), scientific researcher, the Laboratory of Genetics
391105, Ryazan region, Rybnoe district, 26-2. Divovo
L. Khrabrova
FSBSI «ARRI for Horsebreeding»
Russian Federation
Russian Federation
Dr. Habil (Agr. Sci.), Professor, senior researcher
391105, Ryazan region, Rybnoe district, 26-2. Divovo
A. Nikolaeva
FSBSI «ARRI for Horsebreeding»
Russian Federation
Russian Federation
PhD. (Agr. Sci.), senior scientific researcher, the Laboratory of Genetics
391105, Ryazan region, Rybnoe district, 26-2. Divovo
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For citations:
Blohina N., Khrabrova L., Nikolaeva A. The influence of breeding methods on the molecular genetic structure of budenny horse breed. Genetics and breeding of animals. 2019;(2):97-102. (In Russ.) https://doi.org/10.31043/2410-2733-2019-2-97-102
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