Interrelation of polymorphic variants in the promoter of the PRL gene of embryos and egg characteristics for biotechnology
https://doi.org/10.31043/2410-2733-2019-3-3-10
Abstract
Developing chicken embryos (RECs) are used in the cultivation and isolation of viruses in vaccine production programs. The titer of the cultured virus directly depends on the yield of extraembryonic fluid (YEF). Relevant are studies aimed at identifying genetic polymorphism in genes directly related to the formation of chicken embryos. One of the promising candidate genes can be considered the prolactin gene. 420 Russian white breed chicken embryos were genotyped for indel polymorphism (insertion at 24 bp) at position 358 of the prolactin promoter region. 3 genotypes were identified for the prolactin locus: Del / Del- homozygotes for deletion (0,062); In / Del - heterozygotes (0.400); In / In - insertion homozygotes (0.538). Significant differences were found between the genotypes in terms of embryo mass and the incubation rate of incubated eggs (p <0.01). Carriers of the Del / Del genotype were distinguished by higher embryo mass and lowest shrinkage rates. The In / In genotype was characterized by the smallest indicators on the weight of the embryo. Embryos of the Del / Del genotype had reduced shrinkage in comparison with In / In individuals (p <0.01), which indicates their advantageous suitability for obtaining a higher yield of amniotic fluid.
About the Authors
N. Dementeva
Russian research institute of farm animal genetics and breeding — branch of the L.K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
PhD (Biol. Sci.), leading researcher, laboratory of molecular genetics
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
O. Mitrofanova
Russian research institute of farm animal genetics and breeding — branch of the L.K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
PhD (Biol. Sci.)
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
E. Fedorova
Russian research institute of farm animal genetics and breeding — branch of the L.K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
PhD (Biol. Sci.), senior researcher of department of genetics, breeding and conservation of genetic resources of agricultural birds
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
M. Pozovnikova
Russian research institute of farm animal genetics and breeding — branch of the L.K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
PhD (Biol. Sci.), senior researcher, laboratory of molecular genetics
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
A. Azovceva
Russian research institute of farm animal genetics and breeding — branch of the L.K. Ernst Federal science center for animal husbandry
Russian Federation
student St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
Russian Federation
student St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
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Review
For citations:
Dementeva N., Mitrofanova O., Fedorova E., Pozovnikova M., Azovceva A. Interrelation of polymorphic variants in the promoter of the PRL gene of embryos and egg characteristics for biotechnology. Genetics and breeding of animals. 2019;(4):43-48. (In Russ.) https://doi.org/10.31043/2410-2733-2019-3-3-10
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