Interrelation of polymorphic variants in the promoter of the PRL gene of embryos and egg characteristics for biotechnology
https://doi.org/10.31043/2410-2733-2019-3-3-10
Abstract
Developing chicken embryos (RECs) are used in the cultivation and isolation of viruses in vaccine production programs. The titer of the cultured virus directly depends on the yield of extraembryonic fluid (YEF). Relevant are studies aimed at identifying genetic polymorphism in genes directly related to the formation of chicken embryos. One of the promising candidate genes can be considered the prolactin gene. 420 Russian white breed chicken embryos were genotyped for indel polymorphism (insertion at 24 bp) at position 358 of the prolactin promoter region. 3 genotypes were identified for the prolactin locus: Del / Del- homozygotes for deletion (0,062); In / Del - heterozygotes (0.400); In / In - insertion homozygotes (0.538). Significant differences were found between the genotypes in terms of embryo mass and the incubation rate of incubated eggs (p <0.01). Carriers of the Del / Del genotype were distinguished by higher embryo mass and lowest shrinkage rates. The In / In genotype was characterized by the smallest indicators on the weight of the embryo. Embryos of the Del / Del genotype had reduced shrinkage in comparison with In / In individuals (p <0.01), which indicates their advantageous suitability for obtaining a higher yield of amniotic fluid.
About the Authors
N. Dementeva
Russian research institute of farm animal genetics and breeding — branch of the L.K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
PhD (Biol. Sci.), leading researcher, laboratory of molecular genetics
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
O. Mitrofanova
Russian research institute of farm animal genetics and breeding — branch of the L.K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
PhD (Biol. Sci.)
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
E. Fedorova
Russian research institute of farm animal genetics and breeding — branch of the L.K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
PhD (Biol. Sci.), senior researcher of department of genetics, breeding and conservation of genetic resources of agricultural birds
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
M. Pozovnikova
Russian research institute of farm animal genetics and breeding — branch of the L.K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
PhD (Biol. Sci.), senior researcher, laboratory of molecular genetics
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
A. Azovceva
Russian research institute of farm animal genetics and breeding — branch of the L.K. Ernst Federal science center for animal husbandry
Russian Federation
student St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
Russian Federation
student St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
References
1. Stanishevskaya O. I. Organizational aspects of the conservation of genetic resources of farm animals: world experience / O. I. Stanishevskaya, S. V. Cherepanov, Yu. L. Silyukova // Genetics and animal breeding. — 2017. — ‹. 3. — P. 3–11.
2. Lapa M. A. Influence of the genotype of mothers, fathers and the age of developing chicken embryos on the volume and quality of allantoic-amniotic fluid / M. A. Lapa // Genetics and animal breeding. — 2015. — ‹ 1. — P. 14–20.
3. Dementieva N. V. Study of the structure of the gene pool of the Russian white breed of chickens by the method of genomic SNP-scanning / N. V. Dementieva, M. N. Romanov, A. A. Kudinov, O. V. Mitrofanova, O. I. Stanishevskaya, V. P. Terletsky, E. S. Fedorova, E. V. Nikitkina, K. V. Plemyashov // Agricultural Biology. — 2017. — V. 52. — ‹ 6. — P. 1166–1174.
4. Omede A. A. Physico-chemical properties of late-incubation egg amniotic fluid and a potential in ovo feed supplement / A. A. Omede, M.M. Bhuiyan, A.F. lslam, P. A. Iji // Asian-Australasian Journal Animal Sciences. — 2017. — Vol. 30. — ‹8. — ê. 1124–1134
5. Da Silva M. The unique features of proteins depicting the chicken amniotic fluid / M. Da Silva, C. Dombre, A. Brionne, P. Monget, M. Chessé, M. De Pauw, M. Mills, L. Combes-Soia, V. Labas, N. Guyot, Y. Nys, S. Réhault-Godbert // Molecular & Cellular Proteomics. — 2019. — Vol. 15. — ‹ 18. — ê. 174–190.
6. Hinckea å. í. Dynamics of Structural Barriers and Innate Immune Components during Incubation of the Avian Egg: Critical Interplay between Autonomous Embryonic Development and Maternal Anticipation / M. T. Hinckea, M. Da Silvad, N. Guyotd, J. Gautrond, M. D. McKeee, R. Guabiraba-Britof, S. Réhault-Godbertd // Journal of Innate Immunity. — 2019. — Vol. 11. — ‹ 2. — ê. 111–124.
7. Miao Y.-W. Mapping of the prolactin gene to chicken chromosome / Y.-W. Miao, D. W. Burt, I. R. Paton, P. J. Sharp, I. C. Dunn // Animal Genetics. — 1999. — Vol. 30. — P. 470–473.
8. Shimada K. I. H. Expression of prolactin gene in incubating hens / K. I. H. Shimada, K. Sato, H. Seo, N. Matsui // Reprod.Fertil. — 1991. — ‹ 2. — ê.147–154.
9. Dunn I. C. Genetic mapping of the chicken prolactin receptor gene: a candidate gene for the control of broodiness / I. C. Dunn, G. McEwan, T. Ohkubo, P.J. Sharp // British Poultry Science. — 1998. — ‹ 39. — ê. 23–24.
10. Kulibaba R. A. Prolactin and growth hormone gene polymorphisms in chicken lines of ukrainian selection / R. A Kulibaba, A. P Podstreshnyi // Cytology and Genetics. — 2012. — ‹ 46. — ê. 390–395.
11. Cui J. X. Association of Polymorphisms in the Promoter Region of Chicken Prolactin with Egg Production / J. X. Cui, H. L. Du, Y. Liang, X. M. Deng, N. Li, X. Q. Zhang // Poultry Science. — 2006. — ‹ 85. — ê. 26–31.
12. Krutikova A. A. Gene polymorphism of the mstn myostatin, prolactin prl, and dopamine dd2 receptor dd2 drd2 genes in chickens of different directions of productivity. The dissertation for the degree of candidate of biological sciences, St. Petersburg. — 2017, P. 124.
13. Aliev A. K. Development of an inactivated vaccine against infectious bursal disease / A. K. Aliev, O. I. Kiselev, M. G. Aliev, G. R. Yusupova // Uchenye zapiski KGAVM im. N. E. Bauman. — 2011. — ‹ 3. — P. 20–28.
14. Lapa M. A. Influence of various factors on the volume of allantoic-amniotic fluid REC / M. A. Lapa // Proceedings of St. Petersburg State University of Economics. — 2014. — ‹ 37. — P. 65–68.
Review
For citations:
Dementeva N., Mitrofanova O., Fedorova E., Pozovnikova M., Azovceva A. Interrelation of polymorphic variants in the promoter of the PRL gene of embryos and egg characteristics for biotechnology. Genetics and breeding of animals. 2019;(4):43-48. (In Russ.) https://doi.org/10.31043/2410-2733-2019-3-3-10
Views:
363
Email this article 