Acoustic interferometry of erythrocytes with hypercholesterolemia

Abstract

Structural remodeling of erythrocytes in conditions of hypercholesterolemia may be associated with the accumulation of cholesterol in the cell membrane, the end products of lipotoxic intermediate metabolism in cells, which affects on the physicochemical characteristics of erythrocytes and blood viscosity. Structural changes of erythrocytes reflect the severity of pathology. The search for new inexpensive express methods for the diagnosis of dyslipidemia is relevant. The aim of the study was to evaluate, by the acoustic method, the erythrocyte elasticity of rats with alimentary dyslipidemia and intact animals. The study material was whole blood, stabilized with K₃EDTA, plasma and suspension of erythrocytes of rats with hypercholesterolemia. The model of hyperlipidemia was induced in 10 outbred male rats with a body weight of 220-250 g by an atherogenic diet. Erythrocyte elasticity was investigated by acoustic method. The relative content of individual lipid fractions was evaluated by thin layer chromatography. Determination of erythrocyte hematological parameters was made on an automatic analyzer. An increase in the elasticity of erythrocytes in rats with alimentary dyslipidemia has been shown. Indicators of elasticity of erythrocytes depended on the plasma cholesterol and the relative cholesterol content of rat’s erythrocyte. The possibility of using acoustic erythrocyte interferometry in the complex diagnosis of atherosclerosis has been shown.