Variability of the content of serum testosterone in belugas of Delphinapterus leucas of different ages
https://doi.org/10.31043/2410-2733-2020-1-22-26
Abstract
Recently, the issues of a comprehensive study of the reproductive system of marine mammals, in particular the analysis of hormonal changes in the organism of cetaceans in the process of their growth and development has become urgent. In the present work, the content of serum testosterone in blood samples of male beluga whales Delphinapterus leucas was determined. The studies were carried out on belugas kept in semi-free conditions of an open-air marine enclosure complex in the Barents Sea. Venous blood samples were obtained from animals aged 5 to 10 years, while individuals under 7 years old were considered immature. A total of 5 belugas were examined, 43 samples were obtained. Blood sampling was carried out from the ventral or dorsal vein of the caudal fin using a vacuum blood sampling system in plastic vacuum tubes. The volume of the samples ranged from 3 to 12 ml of native blood. Blood serum for research was obtained by centrifuging the samples at 2000 rpm for 10-15 minutes. About 2.5-3 ml of serum was obtained from 8 ml of native blood. It was frozen, stored and transported to the laboratory at a temperature of -20 ° С. The testosterone content in the samples was determined by the method of heterogeneous enzyme-linked immunosorbent assay (ELISA). A comparative assessment of the concentration of this steroid hormone in young and mature animals showed a positive linear correlation between the level of testosterone secretion and the age of the animal. Studies have shown that the sexual maturity of male beluga whales occurs by the age of 8-10, when serum testosterone levels of 15 nmol / L or higher are reached.
About the Authors
N. Inyakina
Federal State Unitary Enterprise «State Research Institute applied problems»
Russian Federation
Russian Federation
senior researcher
191119, St. Petersburg, embankment of the Obvodny Canal, 29
A. Musidray
Russian research institute of farm animal genetics and breeding — branch of the L. K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
graduate student, junior research assistant
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
E. Nikitkina
Russian research institute of farm animal genetics and breeding — branch of the L. K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
PhD (Biol. Sci.), leading researcher
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
E. Mukhachev
Federal State Unitary Enterprise «State Research Institute applied problems»
Russian Federation
Russian Federation
PhD (Biol. Sci.), head of the laboratory
191119, St. Petersburg, embankment of the Obvodny Canal, 29
V. Politov
Russian research institute of farm animal genetics and breeding — branch of the L. K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
researcher
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
G. Shiryaev
Russian research institute of farm animal genetics and breeding — branch of the L. K. Ernst Federal science center for animal husbandry
Russian Federation
PhD (Agr. Sci.), senior researcherSt. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
Russian Federation
PhD (Agr. Sci.), senior researcherSt. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
References
1. Federal law «On the animal kingdom» ‹. 52-FZ dated 24.04.1995 (as amended on 07.05.2003).
2. O’Brien J. K. The value of ex situ cetacean populations in understanding reproductive physiology and developing assisted reproductive technology for ex situ and in situ species management and conservation efforts / J. K. O’Brien, T. R. Robeck // International Journal of Comparative Psychology. — 2010. — ‹ 23. — P. 227–248.
3. O’Brien J. K., Robeck T. R. & Atkinson S. Reproduction. In F.M.D. Gulland, L. A. Dierauf & K. L. Whitman (Eds), CRC Handbook of Marine Mammal Medicine. (3d ed.). Boca Raton: CRC Press. 2018. P. 169–207.
4. Amerkhanov Kh. A. Serum testosterone and cholesterol content in bulls depending on the type of productivity, age and season of the year / Kh. A. Amerkhanov, A. I. Abilov, G. V. Eskin, N. A. Kombarova and others // Agricultural biology. — 2014. — ‹. 2. — P. 59–66.
5. Delville Y. Testosterone facilitates aggression by modulating vasopressin receptors in the hypothalamus / Y. Delville, K. M. Mansour, C. F. Ferris // Physiology & Behavior. Elsevier. — 1996. — Vol. 60. — ‹ 1. — P. 25.
6. Brodie P. F. A reconsideration of aspects of growth, reproduction and behavior of the white whale (Delphinapterus leucas) with reference to the Cumberland Sound, Baffin Island, population / P. F. Brodie // Journal of the Fisheries Research Board of Canada. — 1971. — ‹ 28. — P. 1309–1318.
7. Robeck T. R. Reproduction, growth and development in captive beluga (Delphinapterus leucas) / Robeck T. R., S. L. Monfort, P. P. Calle et al. // Zoo Biology. — 2005. — ‹ 24. — P. 29–49.
8. Montano G. M. Circulating anti-Mullerian hormone concentrations in relation to age and season in male and female beluga (Delphinapterus leucas) / G. M. Montano, T. R. Robeck, K. J. Steinman, J. K. O’Brien // Reproduction, Fertility and Development. — 2016. doi.org /10.1071/RD15537.
Review
For citations:
Inyakina N., Musidray A., Nikitkina E., Mukhachev E., Politov V., Shiryaev G. Variability of the content of serum testosterone in belugas of Delphinapterus leucas of different ages. Genetics and breeding of animals. 2020;(1):22-26. (In Russ.) https://doi.org/10.31043/2410-2733-2020-1-22-26
Views:
400
Email this article 