Assessment of genetic structure of reindeer of the even breed
https://doi.org/10.31043/2410-2733-2020-1-44-49
Abstract
For development of programs for preservation and the description of genetic resources in livestock production use proteins of blood and DNA markers including microsatellites. The domestic reindeers of the Even breed divorced in the Bulunsky district of Yakutia served as object of studying. The polymorphism of serumal protein of a transferrin at domestic reindeers of the Even breed is investigated. It is established that at deer of the Even breed on a locus of a transferrin not the high level of polymorphism is noted, on this locus 16 genotypes were revealed. With high frequency the following genotypes meet: TfC1D1 (22%), TfBD2 (18%), TfC1D1 (16%). At the same time individuals with type аллеля Tfdpo to occurrence frequency authentically surpass animals with other types аллеля (Р <0.001). The method of the DNA analysis determined genotypes of 36 domestic reindeers of the Even breed by 16 microsatellite loci of DNA: (Rt1, Rt6, Rt7, Rt9, Rt24, Rt30, BMS745, BMS1788, NVHRT16, OheQ, C32, C143, C217, C276, FCB193, T40). From 2 to 12 allelic options on a locus are revealed. From 16 studied microsatellite loci at 12 - DNA possess high degree of heterozygosity - from 0.722 to 0.889. The lowest degree of heterozygosity is revealed at loci of C143 and C217 - (on 0.333) and T40 - (0.472). The average of alleles on a locus made 7.75±0.86. High rates (Ai, But, Not, I) loci of Rt1, Rt6, Rt7, Rt9, Rt24, Rt30, BMS745, BMS1788, NVHRT16, OheQ, C32, C276, FCB193 and Tfpozvolyayut to consider these polymorphic loci the genetic markers suitable for assessment of a genetic variety of domestic reindeers.
About the Authors
N. Filippova
State budgetary institution of the Republic of Sakha (Yakutia) «Sakhaagroplem»
Russian Federation
Russian Federation
PhD (Biol. Sci.)
677001, Yakutsk, Ordzhonikidze str., 20
L. Koryakina
Federal public budgetary educational institution of the higher education «Yakut state agricultural academy»
Russian Federation
Russian Federation
PhD (Vet. Sci.)
677007, Yakutsk, St. Sergelyakhskoe highway, 3 km, str., 3
A. Pavlova
Federal public budgetary educational institution of the higher education «Yakut state agricultural academy»
Russian Federation
Russian Federation
Dr. Habil. (Vet. Sci.)
677007, Yakutsk, St. Sergelyakhskoe highway, 3 km, str., 3
Т. Dmitriyeva
Federal public budgetary educational institution of the higher education «Yakut state agricultural academy»
Russian Federation
Russian Federation
graduate student
677007, Yakutsk, St. Sergelyakhskoe highway, 3 km, str., 3
N. Pavlova
Federal state scientific institution «Yakut scientific center of complex medical problems»
Russian Federation
Russian Federation
PhD (Biol. Sci.)
677010, Yakutsk, St. Sergelyakhskoe highway, 4
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Review
For citations:
Filippova N., Koryakina L., Pavlova A., Dmitriyeva Т., Pavlova N. Assessment of genetic structure of reindeer of the even breed. Genetics and breeding of animals. 2020;(1):44-49. (In Russ.) https://doi.org/10.31043/2410-2733-2020-1-44-49
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