Influence of oxidative stress on the transport of bioelements in the small intestine of rats
https://doi.org/10.31043/2410-2733-2020-1-66-72
Abstract
The article studies the features of transport of biogenic cations in the small intestine of rats under glucocorticoid-induced oxidative stress. The processes of ion transport through the gastrointestinal wall were investigated by in vivo perfusion of the small intestine. In perfusion samples, the content of Na, K, Ca, Mg, Fe, Zn, and Cu ions was determined by the method of atomic emission analysis with inductively coupled plasma. The state of the level of redox processes in the small intestine of rats was evaluated by the activity of superoxide dismutase and catalase, as well as the content of malondialdehyde and diene conjugates in tissue homogenates. It has been established that under glucocorticoid-induced oxidative stress in the small intestine, the transport of biogenic macro- and micronutrients is violated - a decrease in the absorption of macronutrients and the secretion of micronutrients. The use of the antioxidant "α-tocopherol" and the new-generation phenolic sulfur-containing antioxidant "Thiofan" in the experiment indicates the important role of free radical lipid peroxidation in the disruption of the transport of macro- and microelements in the small intestine. “Thiofan”, possessing a unique structure that provides intramolecular synergism, protects the membrane structures of cells and helps to correct the transport of bioelements in the small intestine.
About the Authors
S. Lukanina
FSBEI of HE «Novosibirsk State Pedagogical University»
Russian Federation
Russian Federation
PhD (Biol. Sci.)
630126, Novosibirsk Region, Novosibirsk, Vilyuiskaya St., 28
A. Sakharov
FSBEI of HE «Novosibirsk State Pedagogical University»
Russian Federation
Russian Federation
Dr. Habil. (Biol. Sci.)
630126, Novosibirsk Region, Novosibirsk, Vilyuiskaya St., 28
О. Prosenko
FSBEI of HE «Novosibirsk State Pedagogical University»
Russian Federation
Russian Federation
PhD (Chem. Sci.)
630126, Novosibirsk Region, Novosibirsk, Vilyuiskaya St., 28
References
1. Khutsishvili M. B. Free-radical processes and their role in the pathogenesis of certain diseases of the digestive system (part 1) / M. B. Khutsishvili, S. I. Rapoport // Clinical Medicine. — 2002. — ‹ 10. — P. 10–16.
2. Pruchniak M. P., Araźna M., Demkow U. Biochemistry of Oxidative Stress / M. P. Pruchniak, M. Araźna, U. Demkow // Adv Exp Med Biol. — 2015. — Vol. 161. — P. 1–11.
3. Khnychenko L. K. Stress and its role in the development of pathological processes / L. K. Khnychenko, N. S. Sapronov // Reviews on the wedge. pharmacol. and lek. therapy. — 2003. — Vol. 3. — ‹. 3.– P. 2–15.
4. Lee Y. S. Glucosylation of flavonol and flavanones by Bacillus cyclodextrin glucosyltransferase to enhance their solubility and stability / Y. S. Lee, J. B. Woo, S .I. Ryu // Food Chem. – 2017. – Vol. 229. — P. 75–83.
5. Ryazantseva N. V. Typical violations of the molecular organization of the erythrocyte membrane in somatic and mental pathology / N. V. Ryazantseva, V. V. Novitsky // Uspekhi Fiziologicheskikh Nauk. — 2004. — Vol. 35. — ‹. 1. — P. 53–65.
6. Srubilin D. V. Activation of lipid peroxidation processes in the small intestine mucosa in the mechanisms of formation of endogenous intoxication with prolonged intake of dichloroethane / D. V. Srubilin, D. A. Enikeev // Basic research. — 2014. — ‹ 10–9. — P. 1805–1810.
7. Srubilin D. V. The role of the nitroxidergic system in the regulation of oxidative stress in the liver in rats with experimental peritonitis / D. V. Srubilin, D. A. Enikeev, V. A. Myshkin, I. D. Isakov, A. V. Isakova, E. P. Kashirina // Fundamental research. — 2014. — ‹10-4. — P. 724-731.
8. Yakovlev M. Yu. Intestinal lipopolysaccharide: systemic endotoxinemia — endotoxin aggression — SIR — syndrome and multiple organ failure as links of one chain / M. Yu. Yakovlev // Bull. VNTS RAMS. — 2005.– ‹ 1.– P. 15–18.
9. Valeeva I. Kh. Influence of dimephosphon and xidiphon on the lipid peroxidation and antioxidant system of rats treated with prednisone for a long time / I. Kh. Valeeva, L. E. Ziganshina, Z. A. Burnashova, A. U. Ziganshin // Experimental and clinical pharmacology. — 2002. — Vol. 65. — ‹ 2. — P. 40‒43.
10. Binder H. J. Potassium/proton exchange in brush-border membrane of rat ileum / H. J. Binder, H. Murer // J. Membr. Biol. — 1986. — Vol. 91. — P. 77–84.
11. Laihia J. K. Lucigenin and linoleate enhanced chemiluminescent assay for superoxide dismutase activity / J. K. Laihia, C. T. Jansen, M. Ahotupa // Free Radic. Biol. Med. — 1993. — Vol. 14. — P. 457–461.
12. Determination of resistance to oxidation of low density lipoproteins of blood serum: guidelines / Yu. I. Ragina, M. I. Dushkin. — Novosibirsk, 1998. — 11 p.
13. Buslaeva G. N. The value of calcium for the body and the effect of nutrition on its metabolism / G. N. Buslaeva // Consilium Medicum. Appendix ‹. 3 (Pediatrics). — 2009. — P. 4–6.
14. Sidorov A. V. The physiology of intercellular communication. — Minsk: BSU, 2008. — 215 p.
Review
For citations:
Lukanina S., Sakharov A., Prosenko О. Influence of oxidative stress on the transport of bioelements in the small intestine of rats. Genetics and breeding of animals. 2020;(1):66-72. (In Russ.) https://doi.org/10.31043/2410-2733-2020-1-66-72
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