Genetic characteristics of the Pechora type of Kholmogorsky cattle based on microsatellites and DNA markers

The Pechora zonal type (PH-1) was obtained by absorbing crossbreeding of local Northern komolog cattle with the Kholmogorsky breed. Currently, Kholmogorsky cattle are on the verge of extinction, which is due to the widespread metisation of domestic Holstein breeds. The paper presents the characteristics of the allelofond of the Kholmogorsky herd of the Pechora type (n=66) by 11 polymorphic loci of microsatellites. It was found that the polymorphism of tandem repeats in the Pechora type of Kholmogorsky cattle is observed in all 11 loci, the most polymorphic is the tgla53 locus (10 alleles). The most common allele SPS115 248 occurs with a frequency of 0.606. The average number of alleles for 11 loci was 6.2, and the number of effective alleles was 3.4. the obtained indicators were compared with the values published for Yakut [1], Holstein [2, 3] and Ayrshire cattle [4]. It was found that the Pechora type of Kholmogorsky breed has a higher number of effective alleles than Yakut cattle (by 1.0), and lower than other breeds (by 0.3...1.6). Analysis of F-statistics showed that on average, the deviation of the actual heterozygosity from the expected one is insignificant Fis=-0.004, while other breeds, excluding the sample of Holstein bulls, had a more significant excess of heterozygotes. The smallest genetic distance of the studied population was established with the Holstein breed (d=0.221 to cows and d=0.200 to bulls). A more detailed genetic analysis of individual loci showed that the frequency of occurrence of alleles BM2113 Pechora type is closer to the Yakut (r=0.935) and Ayrshire (r=0.875) than to the Holstein breed (r=316...357).

Kholmogorskaya breed, the gene pool of the herd, microsatellites, allele, heterozygosity, genetic similarity

1. Matyukov V. S. Once again about the gene pool and selection of Kholmogory cattle. Syktyvkar, 2007. 139 p.

2. Matyukov V. S. Genetic history and value of the gene pool of the disappearing Kholmogory breed / V. S. Matyukov, Ya. A. Zharikov, N. A. Zinovieva // Dairy and meat cattle breeding. – 2018. – №2. – P. 2-8.

3. Matyukov V. S. Pechora type of Kholmogorsk cattle in the Komi Republic / V. S. Matyukov, V. L. Mikheev // Dairy and meat cattle breeding. – 2005. – №7. – P. 22 -25.

4. Matyukov V. S., Zakharov A. P., Mikheev V. L. [and others]. Breeding and genetic improvement of cattle in the Komi Republic // Syktyvkar, 2003. – 190 p.

5. Methods of modern breeding and preservation of the gene pool of dairy cattle in the Komi Republic: Recommendations for optimization and preservation of the gene pool of Kholmogory cattle / Matyukov V.S., Zharikov Ya. A., Rudometova A.I., et al.: Syktyvkar, 2012. – 157 from.

6. Nikolaev S. V., Konopeltsev I. G., Matyukov V. S. Reproductive qualities of cows of the Kholmogory breed in comparison with other breeds of dairy cattle in the Komi Republic // Modern scientific and practical achievements in veterinary medicine: M. nauch. -practice. conf. Kirov. – 2019. – Issue. 10. – P. 52-56.

7. Nikolaev S. V. Productivity of cows of the Kholmogory breed with varying degrees of Holsteinization in the Komi Republic / S. V. Nikolaev, N. A. Shemuranova // Dairy and meat cattle breeding. – 2020. – №2. – P. 19-23. DOI: 10.33943 / MMS.2020.82.49.005

8. Dotsev A. V. Assessment of the current state of the gene pool of the Kholmogory and black-and-white cattle breeds based on genome-wide SNP analysis / A. V. Dotsev [et al.] // Vavilov Journal of Genetics and Breeding. – 2018. – Issue. 22 (6). – P. 742-747. DOI: 10.18699 / VJ18.418

9. Paronyan I. A. Possibilities of preserving and improving the gene pool of cattle breeds of domestic selection // Achievements of science and technology of the agro-industrial complex. – 2018. – №5. – P. 63-66. DOI: 10.24411 / 0235-2451-2018-10516

10. Dunin I. M., Meshcherov R. K., Kalashnikova L. A. [and others]. Pedigree work with the Kholmogory breed of cattle // Lesnye Polyany. – Volume 33. – 2019. – 72p.

11. Prozherin V. P. The system of selection and breeding work with the Kholmogory breed of cattle in the Arkhangelsk region for the period 2014-2019 / V. P. Prozherin, V. L. Yaluga, T. A. Rukhlova and others // Arkhangelsk. – 2014. – 122 p.

12. Prozherin V. P. Problems of preservation of domestic breeds of dairy cattle / V. P. Prozherin, V. L. Yaluga, L. A. Kalashnikova // Animal husbandry. – 2016. – № 9. – P. 2-4.

13. Sleptsov I. I. Polymorphism of 15 microsatellite DNA loci in Kalmyk cattle and native Yakut cattle bred in the Republic of Sakha (Yakutia) / I. I. Sleptsov, V. V. Dodokhov, N. I. Pavlova [and etc.]. // Livestock and fodder production. – 2019. – Vol. 102. – №2. – P. 60-67.

14. Khabibrakhmanova Ya. A. Genetic polymorphism of Holstein bulls of OJSC "Krasnoyarskagroplem" based on microsatellite DNA markers / Ya. A. Khabibrakhmanova, L. A. Kalashnikova, A. I. Golubkov [and others] // Bulletin of KrasGAU. – 2019. – №3. – P. 135-140.

15. Chasovshchikova M. A. Genetic characteristics of the Holstein breed of cattle using microsatellite DNA markers / M. A. Chasovshchikova // Bulletin of the Orenburg State Agrarian University. – 2019. – №2 (76). – P. 191-193.