Using the KI-67 proliferation marker to evaluate tendon regeneration in rabbits in an experiment
https://doi.org/10.31043/2410-2733-2022-1-41-46
Abstract
The goal is to assess the influence of platelet autoplasm on healing damaged tendons in rabbits.
Materials and methods. The study on the effect of platelet autoplasma on the regeneration of damaged tendon fabric was carried out on rabbits.
In the experiment, rabbits were applied in the field of Achille tendons, since this tendon in animals is injured quite often [1]. Then in the area of damage to the tendon, the experimental group of rabbits was administered tap, while the injured tendons of rabbits of the control group were left without treatment. An intradermal seam was imposed on wounds in the field of leather. A month later, animals of experimental and control groups took samples of tendon fabric in places of damage to immunohistochemical research using the Ki-67 marker, which made it possible to visually demonstrate the action of platelet autoplasm for the regeneration of damaged tendons.
Results. The Ki-67 proliferation marker made it possible to clearly reveal the effect of tap on regeneration during damage to the tendon fabric in rabbits. In damaged tendon tissues in rabbits, proliferative processes go more active than in a healthy tendon. Thanks to the studies carried out, it was established that TAP stimulates proliferative processes in tendon tissues. The proliferation index is higher in the tendon tissues of the experimental group of rabbits, which was administered by TAP than in the control group and in a healthy tendon fabric.
About the Authors
V. Guseva
Saint Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
Guseva V. – PhD (Vet. Sci.)
196084, St. Petersburg, Chernigovskaya st., 5.
T. Kuznetsova
Saint Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
Kuznetsova T. – PhD (Biol. Sci.)
196084, St. Petersburg, Chernigovskaya st., 5.
B. Semenov
Saint Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
Semenov B. – Dr. Habil. (Vet. Sci.); Professor
196084, St. Petersburg, Chernigovskaya st., 5.
References
1. Ivanov A.V. Modern ideas about the mechanisms of reparative regeneration Achilles tendon after its gap / A. V. Ivanov, D. V. Kozlov // Bulletin of the Smolensk State Medical Academy. – 2015. – Vol. 14. – №4. – P. 74-79.
2. Gushchin Ya. A. The effect of fixing liquids on the microscopic structure of small laboratory animals / Ya. A. Gushchin, A. A. Majikyan // International Veterinary Bulletin. – 2014. – №3. – P. 88-95.
3. Ivanov A.V. Analysis of the morphofunctional features of various zones of the tendon complex Fibroblastic phase of reparative regeneration / A. V. Ivanov // Bulletin of new medical technologies. – 2018. – № 3. – P. 166-170.
4. Roshini S. T. Autologous Platelet Rich Plasma for Regeneration of Tendon Injuries in Horses / S. Tina Roshini, A. Arnprasad, B. Justin William, K. Jeyaraja and K. Priyadharshini // Indian J. Anim. RES. doi: 10.18805 / IJAR.B-3653
5. Tracks K. I. Clinical gerontology / K. I. Trackov, A. N. Ilinsky, S. G. Gorline // Georontology. – 2013. – № 4.
6. Pastuh V.V. Prevention of post-traumatic adhesion process around tendons. Dissertation thesis on the degree of candidate of medical sciences. – 2015.
7. Semenov B. S., Guseva V. A., Rybin E. V., Kuznetsova T. Sh., Smooth E. S. The use of platelet autoplasm in the treatment of horses tendon. – Tutorial. – 2018.
8. Ivanov A. A. Index Ki-67 and the type of HSP70 expression in the formation of prognostic groups of patients with papillary cancer of the thyroid gland / A. A. Ivanov, A. M. Avdalan, E. L. Lushnikova // Russian oncological magazine. – 2018. – № 2 (23). – P. 90-94.
9. Lazukin A.V. Factor of proliferation as an indicator of a forecast for breast cancer / A. V. Lazukin // Russian biotherapeutic magazine. – 2014. – №3. – P. 78-82.
10. Calantarley S.S. to the question of determining Ki-67 in biological tissues filled into paraffin / S. S. Calantarly, D. E. Matsko // Bulletin SPbSU. – Issue 1. – Р. 182-186.
11. Dergunova Yu. A. Comparison of immunohistochemical and PCR method for determining the level of expression of Ki-67 in breast cancer tissue / Yu. A. Dergunova, V. V. Ketova, V. K. Bozhenko, S. G. Vardanyan // Bulletin Russian scientific center X-rayoiodiology. – 2018. – №3. – P. 52-68.
12. Winner B. Long-Term Survival and Cell Death of Newly Generated Neurons In The Adult Rat Olfactory Bulb / B. Winner, C.M. Cooper-Kuhn, R. Aigner Et Al. // EUR. J. Neurosci. – 2002. – Vol. 16 (9). – P. 1681-1689.
13. Manyikyan A. A. Features of histological treatment of organs and tissues of laboratory animals / A. A. Majikyan, M. N. Makarova, Ya. A. Guschin // International Veterinary Bulletin. – 2014. – № 2. – P. 103-109.
Review
For citations:
Guseva V., Kuznetsova T., Semenov B. Using the KI-67 proliferation marker to evaluate tendon regeneration in rabbits in an experiment. Genetics and breeding of animals. 2022;(1):41-46. (In Russ.) https://doi.org/10.31043/2410-2733-2022-1-41-46
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