Peculiarities of antenatal development of rat offspring against aqueous extract of centella asiatica

Purpose: study of the characteristics of the antenatal development of the offspring of rats treated with an aqueous extract of centella asiatica as an additional load.

Materials and methods. The experiment was carried out on 126 (90 females and 36 males) white outbred rats, which were divided equally into 9 groups and, in accordance with the group affiliation, as well as the established period, received daily intragastrically an aqueous plant extract of Centella asiatica at a dose of 30 mg/100 g of body weight animal, 1 ml: group 1 - intact animals (control); group 2 - females and males received an aqueous extract of Centella asiatica for 30 days before pregnancy; group 3 - an aqueous extract of Centella Asiatica for 30 days received females before pregnancy, and males - distilled water; group 4 - an aqueous extract of Centella asiatica for 30 days received males before mating, females - distilled water before mating; group 5 - an aqueous extract of Centella asiatica within 30 days before mating and up to 13 days of pregnancy, females received, and males - distilled water; group 6 - an aqueous extract of Centella asiatica within 30 days before mating and from 14 to 20 days of pregnancy, females received, and males - distilled water; group 7 - an aqueous extract of Centella asiatica from 1 to 13 days of pregnancy received females, males - distilled water; group 8 - water extract of centella asiatica from 14 to 20 days of pregnancy received females, males - distilled water; Group 9 - an aqueous extract of Centella asiatica within 30 days before mating and within 20 days of pregnancy, females received, and males - distilled water. Animals of the control group received daily intragastric distilled water, 1 ml. Standard methods were used to obtain dated gestation females. Accounting for the results of the experiment was carried out on the 20th day of pregnancy of rats by euthanasia of animals, the number of live, dead, reserved fetuses was counted, marking the implantation sites, the anatomical structure of the fetuses, their weight and size were evaluated; embryonic (pre- and post-implantation) death of fetuses was considered, anomalies of external and internal development were revealed.

Results. Additional intragastric loading of male and female rats with an aqueous extract of centella asiatica contributed to an increase in the average number of fetuses in a litter, corpus luteum per 1 female, implantation sites, a decrease in pre-implantation death of embryos and overall mortality, as well as an increase in the weight of fetuses and their cranio-caudal sizes, moreover, the duration of the load was expressed in a more positive trend. The load for 50 days was reflected in the highest rates among the studied groups. With regard to the presence of anomalies in the external and internal development of the fetus, an extremely low percentage of individuals with defects should be noted, both in the control and experimental groups.

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