Relationship between mineral metabolism indicators and the level of 25-hydroxycalciferol in the blood of calves in the early post-state period

Purpose: to study the indices of mineral metabolism and vitamin D level in blood of calves and to identify their interrelations.
Materials and methods. To achieve the set objective, blood was taken from the jugular vein of 15 calves aged from 8 to 15 days. Blood serum was analyzed for calcium, inorganic phosphate, magnesium, and alkaline phosphatase activity using standard biochemical test systems and a semiautomatic analyzer CLIMA MC-15. The study of 25-hydroxycalciferol in blood serum was carried out by the enzyme immunoassay method using the test system "25-OH vitamin D-IMAXYZ (IMAXYZ)" and a vertical scanning microplate photometer MULTISCAN.
Results. A different degree of scatter of results was revealed. The highest degree of homogeneity was determined for the calcium level in the blood of calves (CV = 7,57 %). The variation coefficient for the concentration of inorganic phosphorus, magnesium and the calcium to phosphorus ratio is determined within 12,08—16,29 %. The most pronounced heterogeneity in the sample is determined for the alkaline phosphatase (CV = 25,85 %) and 25-hydroxycalciferol (CV = 31,85 %) indicators, in the variation series of which the highest and lowest values differ by more than 2,5 times. Calculation of the Pearson coefficient made it possible to identify the closest relationships between the content of 25-hydroxycalciferol and inorganic phosphate in the blood (r = -0,74) and the Ca / P ratio (r = 0,71). To a lesser extent, the alkaline phosphatase activity (r = -0,61) and the calcium level (r = 0,41) are interconnected with the level of 25-hydroxyvitamin D. Virtually no correlation was found between vitamin D and the concentration of magnesium in the blood.
Conclusion. In calves aged 8 to 15 days, a large spread in the range of alkaline phosphatase activity and 25-hydroxycalciferol content in the blood is determined with a relative homeostatic constancy of the calcium level. The Ca/P indica tor has the closest positive relationship with the 25-hydroxycalciferol level, and the alkaline phosphatase activity shows the closest negative relationship with this indicator, which seems quite logical: insufficient bone mineralization in a growing organism is associated with an increase in the induction of this enzyme. No relationship was determined between the concentration of 25-hydroxycalciferol and magnesium. The data obtained indicate a different state of mineral metabolism in calves in the early postnatal period of life and the need for careful monitoring of metabolism for timely correction of vitamin and mineral deficiency in the diet of growing calves.

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