Test system customization for genotyping the BMP-2 gene 4:28632407 A/G polymorphism in aquacultured rainbow trout

The application of genomic selection in rainbow trout breeding is becoming more feasible with the advent of recent advances in molecular genetics. However, there are significant challenges in the field of aquaculture, as genomic and marker-assisted selection programmes for aquaculture are considerably behind those for terrestrial farm animals. Currently, there is an actual task to develop test systems for genotyping rainbow trout and for efficient enrichment of target populations with favourable polymorphisms. The objective of this study is to develop a test system based on the promising BMP-2 gene and to establish genomic associations between single nucleotide polymorphism (SNP) and the size-weight characteristics of fish.

Materials and methods. The material for the study was comprised of Rofor rainbow trout specimens (n=200), which were evaluated for the following size-weight parameters: fish weight, body length to the end of the caudal fin, length to the end of the scales, head length, height, and body thickness.

Results. The results of the study revealed that the overwhelming majority of fish (98 %) exhibited a heterozygous genotype (AG) while homozygous genotype AA was entirely absent from the sample. The analysis did not identify any notable correlations between size-weight parameters but a potential explanation for this could be the absence of the AA genotype within the population. Nevertheless, the test system was successfully developed and validated. The study of the influence of this polymorphism contributed to the knowledge of rainbow trout genetics. Further application of the developed test system in rainbow trout breeding programs is possible after conducting additional studies to identify economically useful genetic associations in larger samples of individuals.

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