Association of single nucleotide polymorphisms in ghrelin receptor gene with performance traits parameters in chicken

During the recent years there is under way an intensive search for candidate genes, correlated with performance traits in many farm animals, including chicken. To such type of genes belong ghrelin gene (GHSL) and ghrelin receptor gene (GHSR). Ghrelin is a peptide, which influences on appetite. Immediately before feed intake, its concentration is on the highest level, and after feeding it drops down. With the increasing of body weight the levels of ghrelin decrease and this results in lowering of appetite and body weight regulation. For the investigations there were used chicken of two breeds- meat -egg type breed Pushkinskaya from the bioresource farm of VNIIGRZh “Genetic collection of rare and disappearing chicken breeds” (Pushkin, St.Petersburg) and meat bread Cornish (Breeding Company “Smena”, Moscow Region). There were defined genotypes by single nucleotide polymorphism (SNP) rs14678932 with use of PCR-RFLP method. On the base of single factor dispersion analysis there was evaluated impact of the ghrelin receptor gene (GHSR) on the productive traits of hens. There was investigated influence of the genotype with this replacement on body weight in 7, 33 and 90 days, egg mass in 210 and 365 days, egg laying productivity, egg fertility and hatchability. There was found a possible influence of the genotype of hens-layers with SNP rs14678932 gene GHSR on egg performance (P<0,05) and egg mass in в 240 days (P<0,05), but not found a significant influence SNP rs14678932 gene GHSR on body weight in 7 , 33 days and other main productive parameters in Cornish hens. There was not found any associative correlation of SNP rs14678932 gene GHSR with body weight in hens and cockerels of Pushkinskaya breed in 7, 49 and 90 days, but remarked lower body weight in cockerels, homozygous by investigated SNP in GHSR gene.

SNP, GHSR, PCR-PDRF, polymorphism, SNP, ghrelin receptor, GHSR, PCR-PDF, chickens, body weight, productivity

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