Genetic variation of the fragment of the cytochrome oxidase mtDNA of the Siberian chipmunk (Tamias sibiricus)
Abstract
Siberian chipmunk is distributed from the Urals in the east to Chukotka in the west. Siberian chipmunk is one of the most typical inhabitants of the forests of the Magadan Region. Based on the materials collected in June 2016, the genetic variability of cytochrome oxidase mtDNA of the Siberian chipmunk, inhabiting near the city of Magadan, was first studied. The genetic variability of the Siberian chipmunk, inhabiting near the city of Magadan, was first investigated. The nucleotide diversity of six Siberian chipmunks was π = 0.00451 ± 0.0004337, the haplotypic variety Hd = 0.933 ± 0.0498. Five haplotypes were found. By the magnitude of genetic differentiation, all individuals are divided into two groups, one consists of two individuals, the other of four. We can assume that the individuals we studied are descendants of two female founders - we caught the members of two families, founded by two females. Available in the base of IBOL 18 nucleotide sequences in individuals caught in other parts of the range, the parameters of genetic diversity have similar values (π = 0.00326 ± 0.000167 and Hd = 0.843 ± 0.2357). Chipmunks caught in the north-eastern part of China, according to the magnitude of genetic differentiation, represent one cluster, which includes an individual from the Altai Territory. Magadan chipmunks form a separate grouping. The most likely cause of such significant genetic differences is the geographical isolation of Magadan chipmunks from animals from other parts of the range
About the Authors
S. A. Aksyutina
North Eastern State University
Russian Federation
Russian Federation
S. P. Pustovoit
North Eastern State University
Russian Federation
Russian Federation
References
1. Альбертс Б. Основы молекулярной биологии клетки / Б. Альбертс , Д.Брей , К.Хопкин , А. Джонсон , Дж.Льюис , М.Рэфф, К.Робертс , П.Уолтер // М.: БИНОМ. Лаборатория знаний, 2015. - 768 с.
2. Шнеер В. С. ДНК-штрихкодирование видов животных и растений - способ их молекулярной идентификации и изучения биоразнообразия/ В.С.Шнеер В. С. // Журнал общей биологии. - 2009. - Т. 70. - №4. - С.296 - 315.
3. Ratnasingham S., Hebert P.D.N. BOLD: The barcode of life data system (www. barcodinglife.org) / S. Ratnasingham, P.D.N. Hebert // Molecular Ecology Notes. 2007. Doi:10.1111/j.1471-8286.2006.01678.x
4. Жизнь животных / Под ред. В.Е.Соколова. / М.: Просвещение, 1989. - Т.7. Млекопитающие. - 558 с.
5. Маниатис Т. Методы генетической инженерии. Молекулярное клонирование / Т. Маниатис, Э.Фрич, Дж. Сэмбрук // М.: Мир, 1984. - 480 с.
6. Ivanova N.V.An inexpensive, automation-friendly protocol for recovering high-quality DNA / N.V. Ivanova, J.R. Dewaard , P.D.H. Hebert // Molecular Ecology Notes. 2006. - P. 1-5. (doi: 10.1111 /j.1471-8286. 2006.01748.x)
7. Tamura K. MEGA6: MEGA: Molecular Evolutionary Genetics Analysis version 6.0. / K. Tamura, G. Stecher, D. Peterson, A. Filipski, S. Kumar // Molecular Biology and Evolution. - 2013. - 30. - P.2725 - 2729.
8. Librado P. DnaSP v5: A software for comprehensive analysis of DNA polymorphism data / P. Librado, J. Rozas // Bioinformatics. 2- 009. - 25. - P.1451 - 1452.
9. Obolenskaya E.V. Diversity of Palearctic chipmunks (Tamias, Sciuridae) / E.V. Obolenskaya, Mu-Y. Lee, N.E. Dokuchaev, T. Oshida, Mi-S. Lee, H. Lee, A.A.Lissovsky // Mammalia. - 2009. - 73. - P.281-298.
10. Piaggio A.J. Molecular phylogeny of the Chipmunk inferred from mitochondrial cytochrome b and cytochrome oxidase II gene sequences / A.J. Piaggio, G.S.Spicer // Molecular Phylogenetics and Evolution. - 2001. - V. 20. 3. - P.335 - 350.
11. Piaggio A.J. Molecular phylogeny of the Chipmunk genus Tamias based on the mitochondrial cytochrome oxidase subunit II gene / A.J. Piaggio, G.S.Spicer // Journal of Mammalian Evolution. - 2000. - 7. 3. - P.147-166.
12. Lee Mu-Y. Mitochondrial cytochrome b sequence variations and population structure of Siberian Chipmunk (Tamias sibiricus) in Northeastern Asia and population structure in South Korea / Mu-Y. Lee, A.A. Lissovsky, S.K. Park, E.V. Obolenskaya, N.E.Dokuchaev, Y. Zhang, Y. Li, Y.J. Kim, I. Voloshina, A. Myslenko, T.Y. Choi, Mi-S. Min, H.Lee // Molecules and Cells. - 2008. - 26. - P. 566 - 575.
Review
For citations:
Aksyutina S.A., Pustovoit S.P. Genetic variation of the fragment of the cytochrome oxidase mtDNA of the Siberian chipmunk (Tamias sibiricus). Genetics and breeding of animals. 2017;(3):73-77. (In Russ.)
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