Search for genomic associations with sperm quality of Holstein and Black and White bulls
https://doi.org/10.31043/2410-2733-2019-3-3-10
Abstract
One of the important factors affecting the reproductive process is the genotype of the animal. Recently, studies have been actively conducted to identify polymorphic parts of the genome associated with certain traits that may be used in breeding. In our studies, sperm quality assessment of 129 Holstein and black-and-white bulls was carried out. The highest sperm production rates were selected. The maximum doublet volume of the ejaculate varied from 3 to 27 ml, the maximum sperm concentration in the doublet ejaculate was from 0.7 to 2 billion/ml, the total number of spermatozoa was from 2.7 to 26.4 billion. Individual variability was observed in the number of cells with damage of tail and neck (from 0.25 to 6%). Stimulation of respiration by potassium succinate in the semen ranged from 1.0 to 1.3 times, which indicates a different degree of disturbance of membrane permeability. A non-characteristic for fresh sperm, a slight increase in the respiration rate by 2.4 DNP by 1.5 times in some bulls is possible due to impaired spermatogenesis. All the studied parameters were individual variability, which gives an opportunity to search for genetic associations with quality of bull semen. To search for genomic associations a database was formed combining genotypes identified on Illumina Bovine IBDv3 chips and phenotypic traits of sperm quality. Potential SNPs were found for the volume of the doublet ejaculate, motility and the total number of spermatozoa in the doublet ejaculate. No potential SNPs were found for sperm concentration. Selection by genetic polymorphism of genes used as markers will allow selection of bulls with good spermatogenesis and sperm quality at an early age, before physiological maturity and sperm production.
About the Authors
E. Nikitkina
Russian research institute of farm animal genetics and breeding — branch of the L. K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
PhD (Biol. Sci.), senior researcher
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
K. Plemyashov
Russian research institute of farm animal genetics and breeding — branch of the L. K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
Dr. Habil. (Vet. Sci.), leading research scientist
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
A. Musidray
Russian research institute of farm animal genetics and breeding — branch of the L. K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
researcher
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
A. Kudinov
Russian research institute of farm animal genetics and breeding — branch of the L. K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
researcher
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
A. Krutikova
Russian research institute of farm animal genetics and breeding — branch of the L. K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
PhD (Biol. Sci.),senior researcher
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
N. Demetieva
Russian research institute of farm animal genetics and breeding — branch of the L. K. Ernst Federal science center for animal husbandry
Russian Federation
Russian Federation
PhD (Biol. Sci.), senior researcher
St. Petersburg, Pushkin, Moskovskoe shosse, 55a, 196601
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Review
For citations:
Nikitkina E., Plemyashov K., Musidray A., Kudinov A., Krutikova A., Demetieva N. Search for genomic associations with sperm quality of Holstein and Black and White bulls. Genetics and breeding of animals. 2019;(4):9-13. (In Russ.) https://doi.org/10.31043/2410-2733-2019-3-3-10
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