Cryostability effect on drone sperm of synthetic media, egg yolk and honey
https://doi.org/10.31043/2410-2733-2020-1-27-36
Abstract
The search for a diluent that provides physiologically optimal physical and chemical parameters for spermatozoa (pH, osmolarity) and a cryoprotector that can form a vitreous structure without persistent chemical compounds with cell components allows us to achieve significant progress in long-term storage of cells under low-temperature freezing. The questions of cryopreservation of sperm of honeybee drones are considered. The material for research was the sperm of drones of the "Prioksky" breed type of the Central Russian breed of bees, the gray mountain Caucasian and the breed of Karnik bees. The following diluents were tested for cryostability of drone sperm: semisynthetic medium C46 (medium Kakpakov V. T.), nutrient media for insect cell cultures-Lonza Insect-XPRESS, Graces, Schneider's, JPL-41. As cryoprotectors tested - dimethylsulfoxide (DMSO) control, natural honey, honey in combination with glycerine, DMSO, ethanol, methanol. Studies have shown low viability (membrane integrity) (Lim 36,5±1,8% - 39,9±0,3%) and mobility of frozen-thawed sperm (Lim 2,0 - 4,0 points), diluted in synthetic media. Probably the ionic composition of the tested nutrient media with a concentration of hydrogen ions (pH) from 4.6-6.5 differs significantly from the ionic composition of the protoplasm of sperm drones. Semisynthetic medium C46 with the addition of egg yolk and without DMSO does not have cryophylactic properties to protect the life resource of drone sperm. Bee honey, on the contrary, in the course of research proved itself as a cryoprotector, ensuring the preservation of membranes at the level of 37.2±0.5% with a sperm motility of 3.0 points. The highest sperm viability and motility of 4.0 points were observed in a sample with glycerol at a concentration of 3% - 78.0±1.4% and with DMSO at a concentration of 10% - 79.6±1.2%. At the same time, the sperm before freezing was in a state of mesabiosis for 3 months at 3 °C. Thus, the preliminary results of the study for the first time demonstrate the prospects of a non-electrolyte environment for the preservation of the life resource of drone sperm during cryopreservation.
About the Authors
A. Gulov
Federal state budgetary scientific institution «Federal beekeeping research centre»
Russian Federation
Russian Federation
researcher
391110, Ryazan region, Rybnoe, Pochtovayа st., 22
Z. Sayfutdinova
Federal State Budget Scientific Institution «Federal Scientific Centre VIEV»
Russian Federation
Russian Federation
PhD (Biol. Sci.), leading researcher
109428, Moscow, Ryazansky prospect h. 24, b. 1
D. Mitrofanov
Federal state budgetary scientific institution «Federal beekeeping research centre»
Russian Federation
Russian Federation
researcher
391110, Ryazan region, Rybnoe, Pochtovayа st., 22
I. Yazykov
Federal state budgetary scientific institution «Federal beekeeping research centre»
Russian Federation
Russian Federation
junior researcher
391110, Ryazan region, Rybnoe, Pochtovayа st., 22
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Review
For citations:
Gulov A., Sayfutdinova Z., Mitrofanov D., Yazykov I. Cryostability effect on drone sperm of synthetic media, egg yolk and honey. Genetics and breeding of animals. 2020;(1):27-36. (In Russ.) https://doi.org/10.31043/2410-2733-2020-1-27-36
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