Aim: evaluation of the current state of the genetic and allelic diversity of domestic and wild reindeer populations inhabiting the territory of the Republic of Sakha (Yakutia), using STR markers.

Materials and methods. The sample of the domestic reindeer included animals from Even (EVN, n=33), Evenk (EVK, n=31), and Chukchi (Khargin) breeds (KH, n=33). The sample of wild reindeer comprised representatives of the tundra (WLD_TUN, n=119) and taiga (WLD_TGA, n=14) populations.

Polymorphism analysis of 14 STR loci including NVHRT21, NVHRT24, NVHRT76, RT1, RT6, RT7, RT9, RT27, RT30, RT25, RT13, NV03, RT5, and NV73 was performed using proprietary methods on ABI3130xl genetic analyzer (Applied Biosystems, USA).

Allelic and genetic diversity parameters were calculated using GenAlEx software (ver. 6.5.1) and R package “diveRsity”.

To address the genetic structure, Principal Component Analysis (PCA) was performed using the R package adegenet and visualized in the R package gglot2.

The genetic differentiation of reindeer populations was performed based on a matrix of pairwise F_ST values and visualized as a phylogenetic tree using the Neighbor-Net algorithm in the SplitsTree 4.14.5 program.

Results. Analysis of genetic diversity parameters showed that the observed heterozygosity was the lowest in WLD_TGA (H_O = 0.520) among all studied groups and varied from 0.615 in KH to 0.691 in EVK. All reindeer groups were characterized by a heterozygote deficit, as evidenced by the positive values of the inbreeding coefficient: from _UF_IS = 0.101 in EVK to _UF_IS = 0.372 in WLD_TGA. The mean number of alleles per locus varied from 7.1 in EVN to 12.4 in WLD_TUN. Wild reindeer had higher allelic richness (A_R = 7.8–8.6 and A_R = 6.2–6.8, respectively) and number of effective alleles per locus (N_E = 5.3–6, 9 and N_E = 4.1 – 4.5, respectively) in comparison with their domestic relatives. Principal Component Analysis showed that the first Principal Component separated wild reindeer from the domestic reindeer. Analyzing pairwise F_ST values, we found that the KH was the most genetically distant from domestic reindeer breeds (F_ST = 0.072 between KH and EVN, and F_ST = 0.065 between KH and EVK) and from wild reindeer populations (F_ST = 0.076 between KH and WLD_TGA, and F_ST = 0.06 between KH and WLD_TUN). Pairs EVN and EVK (F_ST = 0.047), as well as WLD_TUN and WLD_TGA (F_ST = 0.008) were characterized by insignificant genetic differentiation. Neighbor Net graph showed the formation of two clusters including wild and domestic reindeer populations

Conclusion. The results may be useful for development of breeding programs for breeds of domestic reindeer and conservation programs for wild reindeer.

Purpose: to analyze and establish distribution patterns of QTL associated SNP across common and breedspecific sections of the genome of the Kazakh white-headed and Auliekol breeds.

Materials and methods: Kazakh white-headed and Auliekol breeds were studied. The material for the study is samples of the hair follicles of 712 Kazakh white-headed bulls and 452 Auliekol breeds. Data on animal genotypes were obtained using the DNA chip GeneSeek GGP Bovine 150K. Genome-wide association study (GWAS): calculating linear regression dependence and determination coefficient was performed using Plink. GWAS analysis of four growth indicators was carried out: birth live weight, weaning weight, live weight at 12 months, and average daily weight gain.

Results. The distribution of QTL associated SNP across common and breed-specific sections of the genome of the Kazakh white-headed and Auliekol breeds are uneven. QTL associated SNP were found only on 6 genome sites out of 25 identified by ROH analysis (6 chromosomes common to the two breeds: 3, 5, 6, 14, 20, and 24; 7 breed-specific sites for the Kazakh white-headed – chromosomes: 2, 4, 5, 6, 14 and 26; 12 breed-specific for the Auliekol breed – chromosomes: 1, 5, 6 and 14). Out of 120 QTL associated SNP of the Kazakh white-headed and 49 Auliekol breeds, 37 polymorphic sites get the ROH region. Of these, 36 are QTL associated for the Kazakh white-headed breed and get the areas common to the two breeds. It was found that different polymorphic variants of the same gene (from 2 to 12 SNP) are characterized by unidirectional β values (either positive or negative).

Purpose: Analysis of the first stage of monitoring the genetic variety of two genes of MTDNK of the mixed population of Nerca river. Ola.

Materials and methods. Separate nonsense were caught during the spawning stroke at the mouth of the Ola river in late June - early July 2014 (13 individuals), 2020 (9 individuals) and 2021 (9 individuals). The material (muscles) was stored in a frozen state. DNA allocation and determination of nucleotide sequences of these genes are made in PJSC Sentol, Moscow. The sequences of the nucleotides of the studied MTDNK genes are leveled using the Clustal W program included in the MEGA version of version 11. The specified program was used to assess the value of nucleotide differences (R-disters) between individuals for the design of dendrograms. The clustering method for constructing dendrograms is unnecessary in pairs-group (UPGMA). The quantitative parameters of haplotypic and nucleotide diversity were found using the program DNASP, V. 5.

Results. Nucleotide diversity of 31 sockeye salmon from three samples (2014, 2020 and 2021) from the population of the Ola River for the cytochrome oxidase gene of mtDNA subunit 1 (CO I) was the value π=0.00505±0.000080, haplotypic diversity Hd = 0.708±0.0042.These indicators for 18 individuals from two samples (2020 and 2021) for the cytochrome b gene (cyt b) mt DNA π = 0.00184± 0.000075 and Hd = 0.699±0.0158.Significant differences in the nucleotide sets of both genes between samples of different years were noted. It is possible that the detected interannual differences in the nucleotide sets of samples of sockeye salmon producers are associated with the influence of fish-breeding activities conducted by employees.

Purpose: Studying the genetic parameters of the population of the Northern Deer of the Chukotka breed according to fragments of molecular multi -cosmic analysis of DNA.

Materials and methods. The northern deer, participating in the experiment year -round grazed on natural pastures without additional feeding. The material for the research was the fabric samples (faster of the auricle) of deer of different sexual age groups. Sampling was carried out by a random method from clinically healthy animals during coral work. Analytical work was carried out in the laboratory of DNA technologies of the All-Russian Research Institute of Tribal case using ISSR-PCR method according to the primer (AG) 9C. DNA allocation and PCR production was carried out with generally accepted methods. For calculations, DNA fragments from 180 to 1400 pp were used, clearly distinguishable visually and form pronounced peaks during computer scanning of gels.

Results. Statistically significant differences between populations in the frequencies of ISSR markers were found in the interval of 1 year in 4-7 loci of 11 identified, through generation-from 1 to 4 loci. With an increase in the temporary interval between observations from 1 year to 5 years, changes in the genome by the number of loci and in the frequencies of ISSR markers were not noted. In all cases, the populations of microsatellites identified were in a state of gene equilibrium. Factors stabilizing the genetic structure of the population of the Chukotka breed are free mating characteristic of the northern deer, a significant number of males participating in the reproduction of the herd. Between populations from various ecological and geographical areas of Chukotka, 6 locus (54.5% of cases) have statistically significant differences in the frequencies of ISSR markers. The population in the Arctic tundra area exceeded the population from the forest tundra along short loci (180-210 pp) and (330- 350 pp) at p <0.001, locals of medium length (350-430 pp) <0.05. In turn, the forest-tundra population exceeded the Arctic population in long loci (700-1300 pp) at p <0.001. Ecological and geographical conditions determine the frequency of ISSR markers in the populations of the Chukchi breed, thereby, being a factor in intraspecific differentiation. Considering that the number of Chukotka breed deer is reduced, constant monitoring of the gene pool is needed. The research was carried out with the financial support of the RFBR in the framework of scientific project No. 20-316-90042.

The aim of this study was to identify the effects of highly dispersed silica nanoparticles (HDSns) on the cryoresistance of Ovis Aries cumulus of oocytes in conditions of intra-(IOV) or extra-(EOV) ovarian vitrification.

Materials and methods. Cumulus-oocyte complexes (COC) intended for EOV were treated with solutions of cryoprotectants (CPA) prepared on TC-199 with 10% FBS (fetal bovine serum): CPA-1-0.7 M dimethyl sulfoxide (DMSO) + 0.9 M ethylene glycol (EG);CPA-2-1.4 M DMSO + 1.8 M EG; CPA -3- 2.8 M DMSO + 3.6 M EG + 0.65 M trehalose. The COC was gradually exposed for 30 seconds in CPA-1, 30 seconds in CPA-2, and 20 seconds in CPA-3. At the IOV, the ovaries of the animals were divided into fragments and placed in sterile gauze containers in CPAs prepared on the basis of Dulbecco (PBS) with 20% FBS: CPA-1 - 7.5% EG + 7.5% DMSO (25 min), then in CPA-2 - 15% EG+15% DMSO + 0.5 M sucrose (15 min). 0.001% HDSns (A.A. Chuiko ICP of the National Academy of Sciences of Ukraine) were added to the CPAs and media for thawing of the experimental groups. The straws with the biomaterial were stored in liquid nitrogen for at least 24 hours. The COCs were removed from the straws after thawing and placed successively at 3 min: in a 0.25 M solution of trehalose in TC-199 with 10% FBS at 37°C, then in 0.19 M, and finally in 0.125 M trehalose. COCs from the thawed fragments were sequentially treated with 0.5 M (1 min) and 0.25 M (5 min) solutions of sucrose in PBS with 20% FBS. The final washing of all COCs was carried out in TC-199 with 10% FBS.

Results. The results of morphological (the character of oocyte-cumulus communications) and cytological monitoring (the status of cumulus cells chromatin) revealed a positive effect of 0.001% HDSns on the parameters of cryoresistance of COC both at EOV and IOV. The proportion of cells with compact cumulus in the group of EOV of gametes with HDSns significantly exceeded that in their absence (69 % vs. 51 %, P<0.05). A similar trend was shown in IOV of oocytes (35 % vs. 20 %, P<0.05). The level of apoptotic (TUNEL-test) cells in the group of EOV (44 % vs. 32 %, P<0.05) and IOV (72 % vs. 51 %, P<0.001) of gametes sharply decreased when HDSns were added to cryoprotective media, and the proportion of pyknotic cells decreased during EOV (from 31 % to 21 %, P<0.05) and IOV (from 58 % to 41 %, P<0.05). In general, the obtained results indicate the expediency of using 0.001 % HDSns in the protocols of IOV and EOV of female gametes OVIS ARIAS.

Purpose: to study the effects of a selenic -containing drug on the concentration of selenium in blood serum, organs and tissues of lambs of the Romanov breed.

Materials and methods. In the course of the study, 120 novels of the Romanov breed and 80 lambs obtained from the data of disemeters were used. The formation of control and experimental groups was carried out taking into account the principle of pair analysis. In experiments on assessing the effect of the drug, clinically healthy animals were used on the body. During the study, four groups of lambs were formed at the age of 30 days. The mothers of the first group during the period of suyagnity received the drug “e-sel” at the rate of 1 ml per 50 kg of live weight; The lambs of the first experimental group introduced the drug “E-sel” at the rate of 0.2 ml per 10 kg of live weight according to the instructions. The second group was a control - a physiological solution was administered in the same quantity as the drug with experimental animals; Feeding was carried out by the main diet. The studies were carried out in the period before the chopping (4 months of age) was carried out. Blood fence was carried out from the jugular vein. The concentration of selenium in blood serum lambs was determined by fluorimetrically with 2.3-diaminonaftalin. After the chopping, the lambs were slaughtered. The concentration of selenium in the organs and tissues of the lamb was examined by atomic-adsorption spectrometry.

Results. It was established that the concentration of selenium in the lambs of the first experimental group increased, starting from the 60s from the moment of birth. At the same time, in the lambs of the first experimental group, the concentration increased 2.84 times by the 60th knocks compared to control, 4.08 times compared to control by the 90s from the moment of birth and 8.5 times by 120 -It day from the moment of birth. The concentration of selenium in the kidneys, liver and muscles (the broadest back muscle) of the first experimental group significantly exceeded the values of control. The maximum concentration was noted in the kidneys. No reliable changes were noted between the 1st and 2nd experimental groups in the concentrations of selenium in the kidneys and liver. The concentration of selenium in the liver of the experimental group was 2.94 times higher in comparison with control. In the kidneys, the concentration of selenium was 2.26 times higher for the experimental group in comparison with control. It is noted that an increase in the concentration of selenium in the muscles exceeded control indicators of 4.59, respectively.

Purpose: within the framework of the research work, studies were carried out on the biochemical composition of the blood serum of highly productive cows, different periods of lactation and during the dry period, with tethered and loose housing.
Materials and methods. The object of the study were cows of black-and-white Holsteinized breed with a productivity of more than 8500 kg on the farm. Blood sampling for biotesting was taken from 108 cows before morning feeding. Animals of the experimental groups were formed according to different periods of lactation and during the dry period with different methods of keeping. The study was conducted on the basis of the laboratory of biochemistry and physiology of animals of the SZNIIMLPKh named after A. S. Emelyanov.
Results. In the period from 1-100 days of lactation, there is an increase in calcium from physiological norms by 9.6%, calcium-phosphorus ratio by 32.6 % and phosphorus deficiency by 6.7 %. Fluctuations in the level of calcium in the blood serum from 7.89-12 mg%, phosphorus from 3.02-3.75 mg%. During the peak of lactation, a decrease in the amount of calcium by 2.7 % is noticeable, as well as a calcium-phosphorus ratio by 1.2% an increase in phosphorus by 4.6 %. Within the group, calcium and phosphorus values ranged from 7.13-13.74 mg%, 2.86-4.48 mg%, respectively. During the period of attenuation of lactation, an increase in the content of calcium by 5.3 %, calcium-phosphorus ratio by 10.8%, a slight decrease in phosphorus by 1.3 % is observed. Calcium values range from 8.23-12.32 mg%, phosphorus from 3.02-4.69 mg%. In the dry period, there is a deviation from the reference values of calcium, phosphorus, calcium-phosphorus ratio by 14.4; 6.7 and 8.4 %, re spectively. There is a difference in the level of calcium within the group from 5.82-10.64 mg%, phosphorus from 2.94-4.05 mg%. The level of calcium, calcium-phosphorus ratio in the blood serum of the examined animals in all periods of lactation on loose content are below the norm by 2.3-11.8 % and 6.7-13.6 %, respectively. In the blood of animals of all groups, calcium values range from 4.36-13.45 mg%, phosphorus from 3.06-5.24 mg%.

Purpose: to study the main physiological indicators of newborn piglets in industrial conditions; adaptation based on the obtained data of the generally accepted Apgar scale for intensive production.

Materials and methods. 152 newborn piglets of a Landrace and Duroc mix, obtained from 12 different sows, were studied according to the existing methodology of the adapted Apgar scale in the conditions of an industrial pig farm. The sows from which the piglets were obtained were of different ages, clinically healthy. The sows were fed using the Dry Rapid feed distribution system, the sows had individual feeders, feeding with compound feed. All planned preventive measures (vaccination and deworming) were carried out according to the established schedule. From the first minutes of life, piglets were fed with colostrum and, later, sows' milk by selfsucking of the udder teats, then, for the introduction of complementary foods, on the 10th day, removable group feeders (pre-starter feeding) and nipple drinkers were provided. All piglets selected by us were examined immediately after birth and 5 and 20 minutes after birth. The assessment of their condition was carried out according to the following criteria: assessment of the general physical condition, the presence of visible developmental pathologies, activity, and the intensity of the sucking reflex. Assessment of the general condition, activity and presence of pathologies was carried out by general clinical methods - examination, palpation, auscultation. To assess the sucking reflex of each piglet, a reflexological method was used: a visual assessment of the sucking reflex on the nipples.

Results. In the course of the research, not only the main physiological indicators (parameters, standards) of the above criteria applicable to newborn piglets in industrial conditions have been established, but also the generally accepted Apgar scale for intensive production has been adapted, which will allow you to quickly and reliably assess the morphophysiological state of newborn piglets, their physiological maturity, as well as to make a forecast regarding their further general condition and, based on these signs, to cull.

Purpose. Studying the influence of testosterone on stimulated by somatotropin and theophylline liberation of Ca²⁺ from intracellular depot of oocytes of pigs.

Materials and methods. The material for the studies was oocytes secreted from antral follicles (with a diameter of 3-6 mm) of the ovarian Sus Scrofa domesticus. Oocyte complexes were aspirated from the ovaries at the stage of follicular growth, without signs of visible pathology. The dedicated oocytes were incubated in the modified incubation environment Dulbekko without CaCl₂, containing 36 mg/l of Piruvat NA and 1 g/l glucose. Caicium in the intracellular depot of oocytes of pigs was measured with the help of a chlortetracycline (CTC) fluorescent probe. Oocytes were loaded with a probe for 5 minutes at 370C in an environment containing 40 microns of CTC. Then the cells were washed three times in an incubation environment and transferred to a special quartz glass with cells of 0.05 ml. Dependent on Ca²⁺ fluorescence of the CTC was recorded in oocytes in the environment of Dulbekko. The intensity of the fluorescence of the CTC probe was measured on a fluorimetric installation consisting of a fluorescent microscope, equipped with the necessary light filters and a photometric nozzle of the FMEL-1A. The CTC-Ca²⁺complex-the membrane excited 380-400 nm light, fluorescence was recorded in the area of 530 nm. The intensity of fluorescence was measured in the conc. units. The duration of ultraviolet radiation on oocytes during measurements did not exceed 5 seconds. In all experiments, an EGT was added to the incubation environment at a concentration of 0.5 mm.

Results. It was shown that in the absence of testosterone in oocytes, the addition of somatotropin (bST) or theophylline stimulated the release of Ca2+ from intracellular depot, while their joint action did not lead to an additional exit of Ca2+ from intracellular depot. Inhibition of proteinkinase and did not affect the liberation of Ca²⁺, stimulated separately by bST or Theophylline, as well as their joint action. Against the background of the use of testosterone, the addition of bST or theophylline separately did not lead to the release of Ca2+ from intracellular depot. With the joint action of bST and Theophylline in the presence of testosterone, the liberation of Ca²⁺ from intracellular depot was noted, and the value of this indicator was higher than with the joint action of bST and Theophylline in the absence of testosterone. In stimulated by the joint action of bST and theophylline, the release of Ca²⁺ from the intracellular depot of oocytes in the presence of testosterone is participated in proteinquinase A and microfilaments, since when exposed to proteinquine A and polymerization of cytochalazine microfilaments release of Ca²⁺ from intracellular depots was not recorded.

The present work is devoted to the study of the course of the wound process in animals as well as possible factors influencing it. The topicality of the research in this topic consists in the fact that the experimental results help to increase the survival rate among the animals which have undergone the operation, to accelerate healing, to reduce the organism rehabilitation time, to avoid the bacterial contamination of wounds, purulent diseases and re-infection, to reduce the trauma level in animals, promote the introduction of new efficient pharmacological methods of prevention and suppression of surgical infections, application of laser and ultrasound equipment, discover and use new medicines. The aim of the study. The aim of this work is to conduct a general literature review on the methods of wound management in animals. This review focuses on papers from 2012 to 2022, citing various factors that influence the wound process in order to outline the need for wound management. To make it more manageable and to speed up wound healing in animals, as well as to point out the need for additional wound management models. Conclusion. The use of a complex graft and matrix based on dextran phosphate gel, stem cells and miliacyl has been shown by biological factors to promote healing. A positive alkaline environment has been shown to promote reepithelisation, as it has no adverse effect, in contrast to a strongly acidic environment. In addition, gamma radiation treatment was found to accelerate healing. From this we can conclude that the literature review conducted on this topic indicates the relevance of the area under consideration, and further developments and more in-depth studies are required, as wound processes are a scientific problem that needs to be solved.

Purpose: to study the concentration of selenium in milk and the impact of the introduction of selenium preparations on the concentration of a trace element in milk at various periods of lactation at the Romanovskoye sheep in the conditions of industrial complexes of the North-Western region.

Materials and methods. 80 disemetals of the Romanov breed aged 1 year are involved. Used clinically healthy animals. The diet was compiled taking into account generally accepted recommendations, depending on age and physiological condition. The content of selenium in plants that were used in the diet of animals was determined by fluorimetrically with 2.3-diaminonaphthalin. At the first stage of the study, a group of disemeters was formed after a backed, milk was received by selecting a control milking after 5, 30, 60, 90, 120th day after the start of lactation. The concentration of selenium in milk was studied by atomic-adsorption spectrometry. At the second stage of the study, two groups of disemeters of the Romanov breed aged 1 year were formed. Pregnancy was confirmed by the visual and evaluation method for the lack of hunting in sheep 20-30 days after the derive period, by the lamb-industry and 2.5 months after the shed period by palpation. During the period of pregnancy, the animals of the first experimental group administered intramuscularly drug “E-sel” at the rate of 1 ml per 50 kg of live weight according to the instructions of the drug; The second group, control, introduced a physiological solution in the same concentration as the drug experimental animals. In animals of the experimental and control groups, a study of the concentration of selenium in milk was carried out by selecting a test milking samples after 5, 30, 60, 90, 120th day after lactation.

Results. The concentration of selenium was reduced in various periods of lactation, which is more likely to be associated with a decrease in the total amount of milk produced and the end of the lactation period. In the continuation of the lactation period, the concentration of selenium was lower than reference values. The concentration of selenium in milk was reliably higher in the disetals, which during pregnancy were administered the drug Selena. However, by the second month of lactation, the concentration of the trace element in milk decreased by 47.72 % in relation to the initial period of lactation. It was noted that with the course of lactation and cancellation of the cottage of drugs there is a significant decrease in the concentration of selenium in the milk of the disemets. On the 120th day, lactation concentration of selenium decreased by 5.9 times compared to 5 days of lactation and 4 times for the control group. Thus, it follows that the concentration of selenium in the milk of the disemeters becomes low on the 30th day, therefore, the milk of mothers cannot be a full-fledged source of selenium for growing lambs, since the first, second week after birth was fed exclusively milk, starting from the third week to animals Fertening were introduced, and the lambs were translated to the adult diet only in the ninth week from the moment of birth.

Purpose: The study of biochemical indicators of blood of females and males of fox hybrids with age.

Materials and methods. Research was carried out on females and males of fox hybrids. The age dynamics of changes in the biochemical parameters of the blood was evaluated with a comparative analysis of 2 age groups: females and males of fox hybrids 2 and 5 months (n = 30). The animals were fed once a day (in the morning) with meat mixtures in accordance with age and physiological condition, contained under the same conditions. Blood from the animals was taken into a special test tube with an activator of a clot of lateral subcutaneous vein of the lower leg until morning feeding. They received serum from it by centrifugation at 2000 rpm for 15 minutes. To obtain information about the functional state of the body, biochemical tests were selected that reliably reflect the state of metabolic processes as carbohydrate, lipid protein: general protein, albumins, alanineaminotransferase, aspartaminotransferase, alkaline phosphatase, α-amylase, glucose, urea, creatinine.

Results. Animals in the process of growth and development showed an increase in a number of indicators: urea, creatinine, total protein, albumin and glucose. ALT and AST activity, on the contrary, decreased with age, reaching minimum values 2 in 5-month-old animals. Alkaline phosphatase concentration also decreased with age. The level of α amylase during the growth and development of the animal did not undergo significant changes. Indicators of protein, carbohydrate metabolism, as well as renal markers and enzymes in fox-bearing hybrids are characterized by sex differences. Thus, the biochemical composition of the blood during the growth and development of the animal undergoes a number of changes. The collection and systematization of data will allow the calculation of the reference boundaries of the main blood parameters of lisopesse hybrids to assess the biochemical status of the beast by sex and age.

The indicators of the biochemical analysis of the blood of horses reflect the state of metabolism of the animal and at the same time are potential predictors of the qualitative characteristics of spermatozoa, the freezing of which is one of the most important ways to preserve and further use the genetic material. The aim of this study was to identify correlations between the level of such acute phase blood proteins, as C-reactive protein and fibrinogen, and the qualitative and quantitative characteristics of the native and thawed sperm of stallions. The object of the study was blood and sperm samples from 87 stallions of various breeds, the average age of which was 10.5±0.5 years. To assess the protein status of the blood, in addition to the aforementioned acute phase proteins, the total protein content, the levels of albumin, globulins, and the albumin / globulin ratio were determined. Stallion sperm samples were assessed according to the following indicators: ejaculate volume, concentration, the total number of spermatozoa in the ejaculate, progressive motility and survival at +4°C. When analyzing the correlations between the studied parameters, a statistically significant negative inverse relationship was revealed between the level of fibrinogen in the blood serum and such parameters as the content of progressively motile sperm in native and thawed cryopreserved sperm (r=-0.22, p<0.05 and r=-0.29, p<0.01, respectively), as well as sperm survival in native and thawed sperm (r=-0.48, p<0.001 and r=-0.22, p<0.05, respectively).There was no statistically significant relationship between the level of C-reactive protein and the studied characteristics of the spermogram. The revealed negative correlation between the level of fibrinogen and the characteristics of the stallions’ sperm can be considered as one of the prognostic important indicators that must be taken into account when selecting stallions with the highest indicators of the quality of cryopreserved sperm.

Purpose: to study the influence of the level of active forms of oxygen in native spermatozoa of roosters on the qualitative indicators of freshly exposed and deconed individual ejaculators (mobility, damage to the cell membranes of sperm) and determining the permissible level of AFC generation to improve the cryoponement protocol.

Materials and methods. The object of the study was the roosters of the Rodi-Aland Red (n = 20) breed at the age of 44 weeks of life. All males were kept in individual cells with the “Genetic Collection of Rare and Disappearing Course breeds” of VNIIGRZH systems adopted by the BRK for feeding, posting and light regime. Sperm was received in penicillin bottles with a volume of 10 ml, using the abdominal massage method. They measured the volume of each individual ejaculate, assessed the mobility of sperm, concentration. Cryoconservational was carried out in granules. Thawing of granules was carried out at T 60 ° C in a slit tie. The damage to the plasma membranes of sperm in the native and deconed seed was evaluated using the Suppitial Bluma coloring method. Spermatozoa with damaged membranes was painted red, intact cells remained white (colorless). Each drug estimates at least 200 cells. To determine the levels of AFC generation in spermatozoa of roosters, a method based on luminol-proroxidate hemilyuminescence was used, which was measured on a chemilyuminometer Lum-1200. The time of each measurement was 3 hours, based on the hemiluminiscence of the active form of oxygen (given the growth of the indicator, peak and decline). Cell concentration (7x106 classes/ml) was selected experimentally for measurements, according to the results of a series of preliminary experiments.

Results. As a result of the study, data based on the method of luminol-proroxidate chemilyuminescence for the permissible level of AFC in native sperm of roosters were first obtained. The range of active forms of oxygen (from 75 to 249 volts*sec) has been established, in which cells do not receive significant damage to membrane structures during cryoponservation. In case of exceeding the threshold of 250 volts*S, the number of cells with damaged membranes increases sharply from 17.19% to 62.87%. The data obtained allow the assessment and selection of roosters on the quality of their sperm for the purposes of cryoponservation and the formation of cryobans of reproductive cells.

Purpose: the goal is to study the abundance, distribution area and genetic similarity of regional populations of the Ayrshire breed of the Russian Federation for the possibility of forming a reference population and conducting a genomic assessment using modern methods for small populations of cattle.

Materials and methods. The material was articles and presentations of domestic and foreign researchers, statistical data on the breeding and bovine livestock of the Ayrshire breed of dairy cattle in yearbooks, bulletins and catalogs for evaluating bulls of enterprises in Russia. Studied and analyzed electronic resources.

Results. To introduce genomic selection (GS) for Ayrshire cattle of Russia, it is necessary to form a reference population, which will be used to link molecular markers (SNPs) with the breeding value of animals evaluated using the BLUP method. At the same time, with an increase in the accuracy of the assessment of animals and the accumulation of livestock, the reliability of the genomic assessment increases. Accounting for the productive traits of the descendants and daughters of bulls is necessary to replenish and update the reference population in order to obtain a more accurate genomic assessment in the future. With the introduction of GS, the genetic progress of the population will accelerate, the generation interval and economic costs for the producer will decrease. The accuracy of genomic prediction increases significantly and will exceed the prediction for parental individuals.

Conclusion. As a result of the analysis, it was found that in the coming years it is possible to form a reference population for the genomic assessment of Ayrshire cattle. The reference population will allow as soon as possible to start the process of introducing GS to breed highly productive domestic animals, since today the Ayrshire cattle of Russia cannot compete not only at the world level with the leaders of animal husbandry, but also within the country with imported genetics.

Purpose: comparison of working capacity of service dogs of different breeds depending on their use at different times of the day.

Materials and methods. The studies were carried out on service dogs (n=41) of various breeds: German (18 heads) and Belgian (Malinois) (7 heads) shepherd dogs, Labrador (13 heads), Spaniel (3 heads), used in the following areas: search for explosives and explosive devices, the search for narcotic drugs and substances and the general search profile. The age of the dogs is from 3 to 9 years. Feeding of the entire livestock was carried out with dry complete feed. The effectiveness of the use of service dogs depending on the breed, sex and time of day has been studied. The day was divided into morning - from 6.00 to 12.00, afternoon - from 12.00 to 18.00, evening - from 18.00 to 24.00 and night - from 24.00 to 6.00. Data on the working qualities of dogs in each of the areas are taken from the reporting documents of the "Acts on the use of service dogs" for 2019.

Results. It was found that German Shepherds worked better in the search for explosives in the morning, afternoon and evening - from 0.6 to 0.83% of successful trips, Labradors stood out at night - 1.77%. In the other direction - the search for narcotic substances in the morning, the best result was shown by spaniels - 33.3% of successful trips, in the daytime and at night - by Belgian shepherds - 38.5% and 33.35%, respectively. In the evening – German Shepherds - 44.4%. Comparison of general detective dogs showed no significant differences between breeds. It should be noted that regardless of the breed and direction of application, bitches worked better at night.

Purpose: To assess the degree of emotional stress in clinically healthy dogs of the Husky breed with a different method of fixation, according to the indicators of variational heart rate measurement, in accordance with the indices of R.M. Baevsky. Data indicating a direct dependence on the method of fixation, as well as the method of registration of the electrocardiogram, on the autonomic regulation of the heart rhythm were obtained. Methods for recording an electrocardiogram with the least pronounced effect on humoral and vegetative tone are allocated, the degree of predominance of the interaction of the sympathetic and parasympathetic nervous system in the regulation of the cardiac cycle in dogs is determined.

Materials and methods. To conduct the study, a group of dogs was formed, husky breeds that included clinically healthy 11 dogs with a live weight of 19 to 25 kg. Age restrictions ranged from 2 to 7 years. The electrocardiogram was recorded using the Conan hardware and software complex.

Outcomes. Data indicating a direct dependence on the method of fixation, as well as the method of registration of the electrocardiogram, on the autonomic regulation of the heart rhythm were obtained. Methods for recording an electrocardiogram with the least pronounced effect on humoral and vegetative tone are allocated, the degree of predominance of the interaction of the sympathetic and parasympathetic nervous system in the regulation of the cardiac cycle in dogs is determined.

Conclusion. In the course of the interpretation of the data obtained, it is possible to conclude: the use of electrocardiogram registration techniques using rigid fixation has a more pronounced effect on the vegetative tone, as well as the emotional state of the dogs under study. Taking into account this factor, it should be noted that despite the absence of indications characteristic of diseases in the studied group of animals, variational pulsometry according to the indices of Baevsky R. M. is applicable not only to extract deeper, more valuable prognostic information about the nature of possible changes in the myocardium and coronary vessels under various micro and macro effects on the body, but also to obtain information about the balance and stability of self-regulation processes.