The concentration of recombinant human lactoferrin (rhLF) in the milk of 22 transgenic goats of different ages was studied: the average concentration of rhLF in the milk of goats-producers of the 4th year of lactation was 13.7% higher than that of goats of the 3rd year of lactation (6.07 and 5.24 g/l, respectively).
It was found that the maximum level of recombinant glycoprotein was produced by the mammary gland of animals in the 2nd and 4th quarters of the studied year period. Protein’s concentration in milk measured in the 2nd and 4th quarters was 6.4 g/l and 6.33 g/l for producers of the third year of lactation and 8.14 g/l and 7.02 g/l for goats of the fourth year of lactation, respectively. The correlation between the season of the year and the content of the target protein in milk was detected (p <0.01), it was found that the most active rhLF production in goat milk occurred in late spring and winter periods.
Variability in the content of the protein of interest in milk between individuals was noted. During the study a relatively low level of rhLF was established in animal №0747 - 0.11–2.52 g/l, while its maximum value was detected in the producer № 0403 - 4.52–9.06 g/l (more than 4 times difference). The range of individual variability in terms of rhLF content in the milk of goats of the third and the fourth lactation was 0.11–9.06 g/l.
Milk of genetically modified goats contains 3 times more lactoferrin than human breast milk.
Therefore, rhLF concentration testing in goat milk and tracking the dynamics of this indicator allows us to obtain data that can be used later for breeding pro-grams formation, milk production planning and also to improve the gene pool of transgenic animals.

The aim of this work is to study correlations of the frequencies of SSR markers (Inter Simple Sequence Repeat) and live weight of domestic reindeer. A spectrum of 4 amplicons with a fragment size of 240– 330 bp, 350–430 bp, 440–520 bp and 520–570 bp can be considered specific for the Chukchi breed, since deer of all populations is most common. The variability of SSR markers in populations indicates the similarity between them in most allelic frequencies. This confirms the common origin, economic and breeding use of deer of the Chukchi breed. Loci No. 1, 3, 4, 5, 6 are positively associated with each other, while with other loci they have the opposite correlation direction of communication. For all sex and age groups of deer, loci no. 3 (240– 330 bp), no. 4 (330–350) and no. 5 (350–430) are characterized by a moderately crowded inverse relationship with an indicator of live weight. The average value of the correlation coefficient for these loci was equal to: -0.603; -0.648 and -0.646, respectively. By loci No. 6 (440–520 bp), No. 7 (520–570) and No. 8 (650–690), on the contrary, a direct in action and medium in magnitude connection was noted, the correlation coefficient for these loci an average of 0.266; 0.597 and 0.559, respectively. The data obtained allow us to identify trends in the correlation conjugacy of ISSR markers and live weight, and to reduce the search space for genes whose polymorphism is associated with live weight variability in Chukchi populations. Using the analysis of variance, a reliable influence of the «farm» factor (ecology + farm conditions) on the frequency of occurrence of a DNA fragment with an average value of the influence force η_x² = 65,9% was established. The correlation ratio η_x = 0.811 confirms the presence of a close relationship between the attributes.

Leptin is a hormone produced by adipocytes — cells of adipose tissue, involved in the regulation of eating behavior, affecting the reproductive function, as well as the growth and Constitution of animals. In certain physiological conditions in cows, intensive use of their own fat reserves leads to excessive appearance of volatile fatty acids in the blood, which, in turn, become «satiety signals» and lead to a reduction in feed consumption. At this point, the genetic characteristics of the animal associated with the regulation of metabolism, eating behavior, contributing to or preventing the possible development of ketosis become particularly important. The aim of the research is to determine the possible influence of the leptin locus genotype (y7f, a80v, R25C polymorphisms) on the frequency of ketosis in cattle.
We have genotyped Holstein breeding bulls (PCR/RFLP) belonging to WWS (USA) based on the A80V, R25C, and Y7F loci of the leptin gene, which were evaluated by their daughters and «ketosis resistance coefficients» (Wellness trait index (WT$) ketosis) were determined with a high level of confidence. It was found that the group of breeding bulls with the AARRYY genotype is distinguished by a significantly higher average index of «daughters' resistance to ketosis» compared to the AARCYY and AVCCYY groups, which may indicate that the AARRYY genotype in breeding bulls is one of the genetic factors protecting their daughters from the development of ketosis.
In addition, 150 Holstein cows were genotyped using the same loci, of which 50 had ketosis and 100 were healthy. In the group of healthy Holstein cows, compared with the group of animals with ketosis and hepatopathology, the AARCYY genotype was three times more common.

The article presents the genetic characteristic of the Chukchi reindeer breed. The object of the study was of the Chukchi reindeer. In recent years, the number of reindeer of the Chukchi breed has declined sharply. Reduced reindeer numbers could lead to biodiversity loss. The Chukchi breed of deer has good meat qualities, has high germination viability and is adapted in adverse tundra conditions of Yakutia. Herding of the Chukchi breed of deer in Yakutia are engaged only in the Nizhnekolymsky district. There are four generic communities and the largest of which is the agricultural production cooperative of nomadic tribal community «Turvaurgin», which was chosen to assess the genetic processes of breed using microsatellite markers: Rt6, BMS1788, Rt 30, Rt1, Rt9, FCB193, Rt7, BMS745, C 143, Rt24, OheQ, C217, C32, NVHRT16, T40, C276. It was found that microsatellite markers have a wide range of alleles and generally have a high informative value for identifying of genetic differences between animals and groups of animal. The number of identified alleles is one of the indicators of the genetic diversity of the population. The total number of detected alleles was 127. The Chukchi breed of deer is characterized by a high level of heterozygosity, and the random crossing system prevails over inbreeding in the population. On average, there were 7.9 alleles (Na) per locus, and the mean number of effective alleles (Ne) was 4.1. The index of fixation averaged 0.001. The polymorphism index (PIC) ranged from 0.217 to 0.946, with an average of 0.695.

In modern poultry farming, along with classical breeding methods based on phenotype analysis, it is possible to use MAS breeding, which includes genotype assessment. Meat productivity is influenced by live weight and body characteristics of the bird. This article analyzes the influence of the rs316247861 marker of the myostatin gene on the conformation parameters of Pushkin breed chickens. For the analysis, 140 heads of Pushkin breed chickens were taken. Exterior assessment was carried out in chickens at the age of 330 days according to 17 indicators: body weight, body length, body + neck length, oblique body length, thigh length, chest depth, chest width at collarbones, pelvic width, chest girth, metatarsal girth, shin girth , chest angle (degree), keel length, metatarsal length, tibia length, oblique body length with a tape (KDT), tibia length with a tape.
PCR-RFLP analysis was performed using the restriction endonuclease BstHHI enzyme. As a result of the study of the experimental population of Pushkin breed chickens, carriers of the CC genotype were significantly superior to individuals with the GG genotype in terms of indicators affecting the formation of poultry meat productivity. In the sample of chickens of the studied population, the value χ₂=10.29, which allows us to conclude that the genetic equilibrium is shifted, this is a sign of strong selection pressure. Thus, in the experimental population of the Pushkin breed, the CC genotype is desirable for selection for poultry meat productivity. In this regard, the MSTN gene and its expression product deserve special attention, among the issues of both molecular genetics and animal husbandry.

The article presents studies of the Holstein-Kholmogorskaya cattle in the Murmansk region conducted during the period from 2008 to 2017. Traits of the exterior, milk productivity and quality indicators of milk, lactation dynamics, reproductive traits, calving, culling and predisposition to animal diseases were studied. Some problems were identified related to the main traits characterizing a stock of cattle. Reproductive problems were shown up. Calves per 100 cows were 76-78 heads in different years, the fertility rate was 48%, the average insemination index was 2.05, the interval from calving to 1 insemination — 87.9 days. The period of economic use of cows is reducing. The quality traits of milk are deteriorating (milk fat content — 3.3%, protein — 2.95%).
The main directions of further improvement of Holstein Kholmogorskaya cattle in the Murmansk region are identified: an improvement of reproduction traits, an increase of the period of economic use and an increase of the level of mass fraction of fat and protein in milk.
Target indicators of economically useful traits are determined. Upgrading these characteristics to the target level will significantly improve the quality characteristics of livestock.
In reproduction, the increasing calves per 100 cows to 80 heads and the fertility rate of cows at the first insemination to 60%, the reducing the insemination index to 1.5, the decreasing the calving interval from calving to 1st insemination by 8 days (to 80 days), and endeavour to reduce the frequency of reproductive diseases, the number of stillborn calves, to increase the number of twins, the alive of calves at an early age.
The decreasing of the frequency of lameness, ketoses, paresis and other metabolic diseases, mastitis and reproductive diseases, and the improvement of the exterior traits (udder, limbs, and pelvis) will increase in the longevity of cows.
The improvement of the level of animal feeding and balancing of rations will increase (to target values) in fat content (4.0%) and protein (3.1%) in cow's milk with maintaining high milk productivity. It is necessary to continue the selection to improve the exterior performance of the udder. It is also necessary to apply veterinary measures to reduce the incidence of cow mastitis.

Ensuring food security of the country and individual regions is an important state task. In the Magadan Region the consumption of meat and meat products per capita per year is 68% of the recommended norm. In the structure of meat production, cattle occupy 16.4% of the total volume (or 107 tons). Where in the region have the necessary conditions for the development of beef cattle breeding. The relevance of the study is determined by the ability to predict indicators characterizing the meat productivity of livestock. The work was carried out to establish the relationship between exterior development and economically valuable traits of cross-breeding animals obtained by breeding dairy cattle with producers of meat breeds (Hereford, AberdeenAngus) in the circumstances of the Magadan Region. Correlation analysis revealed a strong reliable interdependence between live weight at 17 months with height at the withers and sacrum at 4 months age in groups of second-generation crossbred gobies with a correlation coefficient from 0.74 to 0.85. A positive relationship of varying strength (from weak to medium) of live weight with the measurements of the chest in all four experimental groups was established. The paired linear regression coefficients found during the regression analysis allow us to conclude that the most important criteria for selecting bulls for meat fattening are height measurements at the withers and in the sacrum. Models of multiple linear regressions describing the relationship of live weight and slaughter indicators with the exterior measurements of animals are constructed to determine the complex influence of factors.

The paper presents materials on the rearing of Siberian sturgeon of the Lena population (Acipenser baerii Brandt), sterlet (Acipenser ruthenus Linnaeus) and their hybrid sturgeon (Acipenser baerii Brandt × Acipenser ruthenus Linnaeus) in the conditions of natural temperature regimes of natural reservoirs of the Republic of Karelia. It is noted that the climatic conditions of the region allow us to grow sturgeon to marketable sizes in cages in the natural temperature ranges of natural reservoirs. In order to determine the most suitable object for growing, the fish-breeding, biological and morphological indicators of Siberian sturgeon, sterlet and hybrid sturgeon were studied. The results of growing these objects in the cages of the Kedrosero fish hatchery showed their prospects for use in commercial fish farming. The highest indicators were observed when growing Siberian sturgeon, which showed a significantly higher rate of weight growth, good survival and effective use of feed. When grown for three years, the weight of Siberian sturgeon was 2053 g, sterlet — 682 g. The growth rate of the hybrid sturgeon was lower than that of the Siberian sturgeon and higher than that of the sterlet. The assessment of plasticity and exterior indices also showed the advantage of the Siberian sturgeon in comparison with other species. Fish-breeding, biological and morphometric indicators of sterlet and sturgeon hybrid were some lower, however, the high taste and nutritional value of these species make it possible to consider them quite promising objects of cultivation in the Northern regions.

The research was conducted in order to study the breeding value of bulls-producers of various origins, used in the breeding stock of cows of the Yaroslavl breed in the Vologda region. The work was done on the basis of the information analytical system «Seleks» for 52 bulls-producers of Yaroslavl and Holstein breed obtained in different countries of the world and used the herd of 1600 cows dairy cattle breeding farm ZAO Sheksna Vologda region. General scientific research methods were used: monographic, statistical, and data visualization. It was found that the used bulls were produced in 5 countries of the world: the USA, Canada, Germany, the Netherlands and Russia. The vast majority of 76.9% of used manufacturers are of domestic origin. It was determined that in the controlled population, the factor «country of origin of the bull» has a significant influence (P<0.001) on the breeding value of producers based on the maternal ancestry (η²=0.46). Bulls from Canada and the United States have the highest indicators of breeding value for the milk yield of their maternal ancestors — 15436 and 14210 kg of milk, respectively. Domestic bulls have a breeding value of 8310 kg of milk, which is 7126 kg less than that of producers from Canada (P ≤ 0.001). The best indicators of bull potential realization by maternal ancestry were found in the daughters of Dutch and Russian producers (64.1% and 69.5%, respectively). The higher-than-average yield for the herd is obtained from cows with a Holstein blood type of 50% or more, using purebred Holstein bulls, both foreign and domestic selection, as well as Yaroslavl bulls with a high degree of blood type for improving breed (75-88%). In order to preserve the Yaroslavl breed of cattle, it is necessary to determine the optimal degree of blood transfusion of the Holstein breed, taking into account not only the productive characteristics, but also the characteristics of the health and longevity of animals.

Research is focuses on the possibility of using udder dairy composite (UDC) and feet and legs composite indices (FLC) as indicators of the reasons for the culling of Ayrshire cows based on the results of their exterior assessment after the first calving. The processing included data on the exterior assessment and the reasons for their cull of 855 first calving cows.
Almost all the correlation coefficients of indices with the classification assessment of exterior features were positive and reliable. The UDC is most correlated with the classification of the udder (0.614***) and the total score (0.535***), the least — with the dairy strength (-0.126***). The FLC is highly correlated with the classification of legs (0.940***), with the total score(0.529***), low negative and unreliable with a dairy strength (-0.033). The indices are interconnected at 0.223***. The ranking of UDC and FLC by level revealed an incomplete coincidence of the point complex assessment of some signs of the exterior. The ranking of UDC by level revealed an increase in the scoring classification of all signs of the exterior, the FLC has a mismatch. In culling cows due to limb diseases the FLC was negative (-0.44 ± 0.31) and low compared to other groups. The value of UDC with diseases of the udder is -0.06 ± 0.08. In other animals, these were neutral or positive values. Cows with negative values of both indices were identified in each group of diseases. For example, in groups with udder disease and metabolic disorders, the number of such cows is almost the same 26.0 and 26.3%, and with diseases of the reproductive organs and legs — 20.7 and 22.2%. The force of influence of the indices on retirement is η²UDC=42.0***% (F=7.6) and η2FLC=40.0***% (F=13.2). Studies have revealed predicted retirement rates with UDC and FLC data.

The aim of the work is to study the productivity of Large White gilts that were estimated and selected for herd replacement in the process of line formation. Within 6 generations of selection the age of live weight 100 kg achievement reduces on 6 days, fat thickness on 1,7–2,0 mm, the depth of longissimus muscle increases on 4,9 mm and lean meat output in carcass on 1.4%. The size of litter of dams in selection group increases up to 13,0 piglets, the number of pigs at weaning — to 11,4, the litter weight at 30 days — to 92,4 kg. The productivity increase of replacement pigs is caused by though not high but reliable hereditability indices, calculated according to results of dispersion analysis of data's of 968 gilts, selected from 3 sires groups and 6 dams groups of adjacent generations F₂–F₃, F₃–F₄, F₄–F₅. The influence of sires (factor A) is maximum for age at 100 kg weight (h²=36,5%) and for lean meat output (h²=3,3%) in dams group F₃–F₄; the fat thickness over 6–7 pectoral ribs (h²=4,8%) and the depth of longissimus muscle (h²=5,4%) in dams group F₂–F₃. The influence of dams (factor B) is low in all groups. The mutual influence of both factors apparent in dams group F₃–F₄ in fat thickness in three points of scanning (h²=3,8-7,9 %) and in longissimus muscle depth (h²=2,6%); in dams group F₄–F₅ — in 100 kg weight age (h²=6,7%) and in fat thickness over the 6–7 pectoral ribs (h²=7,0%).

The research was carried out on 2 groups of Holstein cows-analogues in productivity, age and calving times in the transit period. One was a control, the other an experimental one. In addition to the main diet, 150 ml of liquid energy feed based on propylene glycol and glycerol was added to the cows of the experimental group two weeks before calving and 250 ml per day after calving, with the addition of dry microalgae Spirulina Platensis and the antioxidant dihydroquercetin, in a concentration of 0.1% by weight (EBA FA).Inclusion in the diet of cows EBA FA. it had a positive impact on milk productivity, chemical and physical properties and sanitary and hygienic condition of milk. In cows of the experimental group in the 1st and 2nd month of lactation, the average daily milk yield was 26.8 and 29.0 kg with a mass fraction of fat 3.74 and 3.78%, protein 3.32 and 3.07%, including true 3.10 and 2.85% and casein 2.57 and 2.42%; lactose 4.78 and 4.88% and the mass fraction of dry matter 12.72 and 12.51%, respectively, while in the control group cows they were equal in average daily milk yield of 24.0 and 26.5 kg, the mass fraction of fat 3.91 and 3.74%, protein 3.16 and 3.18%, including true 2.95 and 2.86% and casein 2.47 and 2.40%, lactose 4.80 and 4.88% and the mass fraction of dry matter 12.81 and 12.49%. Milk of cows of the experimental group was characterized by lower oxidation and better ORP, lower content of acetone and β-oximabutyric acid. The inclusion of EBA FA in the diet of cows had a positive effect on cows udder health as evidenced by a decrease in the content of somatic cells in the milk of cows of the experimental group by 25.2 and 50.2% in relation to the control, respectively, at the 1st and 2nd month of lactation.
EBA FA also influenced the composition and dynamics of the content of fatty acids in milk. The fatty acid composition of the milk of cows of the experimental group at the 1st month of lactation was characterized by a lower content of myristic, palmitic, stearic and oleic acids and was characterized by a high content of polyunsaturated acids and transisomers. At the 2nd month, the content of oleic acid increased and the content of monosaturated acids increased .compared to the control.

The article is devoted to determination of the new drug Aldiecoseptimol (Patent 2562929) effectiveness for the prevention of the after calving complications of gynecological diseases in first-calf heifers, with the calculation of the values of the service period, its impact on the level of reproduction and economic efficiency in comparison with the control. The work was carried out in highly productive herds of the Moscow region with a daily productivity of 35-40 liters of milk, with completed lactation (305) days of 7200-8000 kg of milk. The effectiveness of prevention was evaluated based on the results of the actual service period in the experimental (n=113) and control (n=102) groups as follows: the service period is less than 85 days — the surplus group, SP-85 days-optimal, SP-86-120 days-deficient, SP-121-135 days or more-acutely deficient in the level of reproduction and output of calves. It was found that after preventive treatments, the number of the first-calf heifers with insemination efficiency of up to 85 days increases and makes up 40% of their total number in the experiment compared to 22% in the control (18% in favor of experience). There was a significant reduction in animals with a service period of more than 121 days: from 60% in the control to 30% in the experiment (acutely deficient group). In the experimental group, compared with the control group, the indicator of the level of reproduction and output of calves increased. The obtained data were processed in accordance with a mathematical model developed by us to optimize the planning of the reproduction process and evaluate the economic efficiency of using a new drug. It was found that the new Protocol for processing first-calf heifers in the of after calving period for the prevention of gynecological diseases contributed to an increase in the number of animals in surplus, in terms of reproduction and output of calves, from 22% in the control to 40% in the experiment. The number of animals with an acute service period decreased from 60% in the control group to 30% in the experimental group. The calculated yield of calves in the experimental group increased on average from 79.4% in the control to 95.4% in the experiment per 100 cows, which was 20.2%. The reproduction rate also increased by 20.2% and amounted to 46 heifers per 100 head of cows in the experimental group instead of 38 heifers per 100 head of cows in the control group. Protocol using the new drug Eldecanato proposed for prevention of after calving complications and gynecological diseases in cows, reduced service period and days's barrenness at 77.6 days, and contributed to the increase of output of calves per 100 cows by 20.2%. All this were permitted to increase economic efficiency in the experimental group by 17.7 thousand rubles per 1 cow per year

A growth in the number of deer and an increase in their productivity are closely related to the solution of the complex problem of reproduction and rational use of deer females. Significant damage to reindeer husbandry is caused by the barrenness of northern domestic deer, the level of which has been quite high in recent years. So, in the Republic of Sakha, on 01.01.2020, the number of domesticated reindeer was 152,068, of which female deer and heifers — 71,818, offspring per 100 females — 59, business yield amounted to 52 fawns per 100 females. The causes of infertility are very diverse, as the natural and climatic conditions of the breeding zones have a significant influence on the reproductive function of reindeer. In Yakutia, the main population of northern domestic deer is bred in mountain taiga and tundra natural-climatic zones, so there are about 55 thousand deer in the mountain taiga zone and more than 65 thousand deer in the tundra zone. In this regard, the study of the peculiarities of the postpartum period course in domestic reindeer females, its morphophysiology, and behavioral reactions depending on the natural and climatic conditions of breeding is of great importance.

The hematoophthalmic barrier is one of the mechanisms of resistance that serves to protect the body and prevents homeostasis disorder when the body is affected by factors that can disturb this balance. One of the complications of mechanical trauma of the eye and violation of the hematoophthalmic barrier is the appearance of oxidative stress on the background of the general inflammatory process in violation of cellular structures. Objective: to study the dynamics of catalase activity in the heart and muscle tissue of rats under oxidative stress caused by mechanical action on the hematoophthalmic barrier. Materials and methods. The study was carried out on white neonatal mature healthy male rats of six months of age, weighing 200–220 g and having 150 pieces. The activity of catalase in heart and muscle tissue was studied before the beginning of the experiment, as well as on the 1st, 3rd, 5th, 7th and 14th day of the experiment using the standard Korolyuk M.A. method. The dynamics of catalase activity was evaluated by different methods of treatment of mechanical eye trauma, including the use of quercetin antioxidant. The results of the study. It was found that in addition to the standard therapy of mechanical eye injury injection of quercetin, the activity of catalase in the heart and muscular tissue after falling on the background of induced oxidative stress increases to the values of physiological norm by 14 days, which indicates the effective elimination of oxidative stress. Conclusions. The activity of catalase in rat heart and muscle tissue under oxidative stress caused by mechanical influence on hematoophthalmic barrier is most effectively stabilized under standard therapy of mechanical eye injury with addition of quercetin in the form of injections.

Current trends in the development of new drugs are based on the creation of nanostructured compositions with improved characteristics. One of the approaches to creating a nanoscale form of drugs, in particular 20-hydroxyecdysone, is the synthesis of its clathrate complex with the clathrate-forming arabinogalactan. Thus obtained nanoscale form of 20-hydroxyecdysone provides an improvement in its physico-chemical properties, which leads to increased absorption, increased bioavailability and, ultimately, to reduce therapeutic doses. In the present work, the effect of the created nanoscale form of 20-hydroxyecdysone on the function of the antioxidant defense system, natural resistance, growth and development of Sovetskaya Chinchilla rabbits was studied for the first time. It has been established that the nanoscale form of 20-hydroxyecdysone, when administered to rabbits at doses of 1.0 and 5.0 mg / kg body weight, contributes to a statistically significant increase in serum BASK and LASK levels, increased activity of the enzymatic unit of the antioxidant system, in particular SOD and catalase in erythrocyte lysate. On the contrary, the content of MDA in the blood plasma was statistically significant [P≤0.05 — P≤0.02] was lower in rabbits of the experimental groups compared with the control, which may indicate a decrease in lipid peroxidation processes in animals under the influence of nanoscale form 20-hydroxyecdysone. In the rabbits of the experimental groups, compared with the control, they were statistically significantly higher by 32-38% [P≤0.05–P≤0.01] absolute and average daily weight gain of rabbits. The optimal dose of the nanoscale form of 20-hydroxyecdysone is dose 1, 0 mg / kg rabbit body weight.

In these studies, a comprehensive assessment of the effect of the drugs «Carofertin» and «Hemobalance» on the reproductive function of oocyte donor cows was carried out. At the same time, the effect of these drugs on the biochemical parameters of blood serum, as well as on the concentration of progesterone, carotene in the experimental groups and the activity of the ovaries in the experimental animals was assessed. An increase in the activity of alkaline phosphatase in the first and third experimental groups was found, which amounted to 128.80 and 104.6 IU / L. There was also a decrease in the concentration of cholesterol in the experimental groups compared with the control. The maximum concentration of carotene was noted in the experimental group No. 1, which were injected with «Carofertin» subcutaneously, and in the experimental group No. 3, which were injected with «Carofertin» and «Hemobalance». The maximum concentration of progesterone was also noted in groups No. 3 and No. 1 and amounted to 21.98±3.03 and 17.01±9.68 nmol / l, respectively. In the course of ultrasound examination of the ovaries in experimental animals, a relationship was noted between the concentration of progesterone and the intensity of the blood supply of the corpus luteum on the ovaries in experimental animals. Subsequently, in one animal from each experimental group, oocytes were aspirated (OPU) twice with an interval of two weeks and their qualitative and quantitative characteristics were assessed. The maximum number of oocytes suitable for use was noted in the experimental group No. 3, which was injected with both study drugs.